一步一步追踪RNA聚合酶。

Tracking RNA polymerase, one step at a time.

作者信息

Vassylyev Dmitry G, Artsimovitch Irina

机构信息

Department of Biochemistry and Molecular Genetics, University of Alabama, Birmingham, Alabama 35294, USA.

出版信息

Cell. 2005 Dec 16;123(6):977-9. doi: 10.1016/j.cell.2005.11.030.

DOI:10.1016/j.cell.2005.11.030

PMID:16360025

Abstract

Although the structural analysis of transcription has attained atomic resolution, techniques to manipulate single molecules involved in transcription have lagged behind. A recent paper in Nature by Abbondanzieri et al. (2005) closes this gap. This study finds that individual molecules of bacterial RNA polymerase move in single base-pair steps and concludes that RNA polymerase acts as a Brownian ratchet that is driven forward by the binding of incoming nucleoside triphosphates.

摘要

尽管转录的结构分析已达到原子分辨率，但用于操纵参与转录的单个分子的技术却滞后了。阿邦丹齐耶里等人于2005年发表在《自然》杂志上的一篇近期论文填补了这一空白。该研究发现，细菌RNA聚合酶的单个分子以单碱基对步长移动，并得出结论：RNA聚合酶起着布朗棘轮的作用，由进入的核苷三磷酸的结合向前驱动。