Rizk Diaa E E, Hassan Hazem A, Ramadan Gaber A, Shafiullah Mohamed, Fahim Mohamed A
Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates.
Urology. 2005 Dec;66(6):1343-8. doi: 10.1016/j.urology.2005.06.113.
Urinary and fecal control deteriorates after menopause, but it is not clear whether this is age or hormone related. This study investigates whether administration of estrogen and/or the anti-aging growth hormone-releasing peptide, ghrelin, improves the adverse effects of menopause/aging on urethral and anal canal submucosal blood vessel counts in middle-age rats.
Female Wistar rats (13 months old) underwent ovariectomy, followed 1 month later by intraperitoneal once-daily administration of 17-beta estradiol (10 microg/kg), ghrelin (2 microg/kg), both hormones, or vehicle (n = 6 in each of four groups) for 42 days. An age-matched sham group (n = 6) received no intervention. Submucosal blood vessels were counted by light microscopy in five randomly selected fields from five nonconsecutive sections (5 microm thick) per rat of formalin-fixed and paraffin-embedded tissue blocks of the urethra and anal canal stained with hematoxylin-eosin. The results are expressed as the mean vessel number per high power field (x400).
Ovariectomy significantly reduced submucosal urethral and anal vascular counts below the sham values (7.41 +/- 0.98 versus 5.46 +/- 0.82, P = 0.003 and 7.16 +/- 1.11 versus 4.92 +/- 0.65, P = 0.0009, respectively). Estrogen restored the urethral counts (7.76 +/- 0.88, P = 0.5) and ghrelin or combined estrogen and ghrelin administration significantly increased the counts to greater than the sham counts (8.68 +/- 0.99, P = 0.04 and 9.72 +/- 1.21, P = 0.004, respectively). Estrogen, ghrelin, and combined estrogen and ghrelin administration also restored the anal counts to sham levels (7.26 +/- 0.97, P = 0.8; 6.56 +/- 0.78, P = 0.3; and 7.76 +/- 0.88, P = 0.3, respectively).
Combined or individual replacement of estrogen and ghrelin produces a beneficial effect by reversing the ovariectomy-induced decrease in urethral and anal canal submucosal vessel numbers in middle-age rats.
绝经后泌尿和排便控制能力下降，但尚不清楚这是与年龄相关还是与激素相关。本研究调查给予雌激素和/或抗衰老生长激素释放肽胃饥饿素是否能改善绝经/衰老对中年大鼠尿道和肛管黏膜下血管数量的不良影响。
13月龄雌性Wistar大鼠接受卵巢切除术，1个月后腹腔内每日注射17-β雌二醇（10微克/千克）、胃饥饿素（2微克/千克）、两种激素或赋形剂（四组每组n = 6），持续42天。年龄匹配的假手术组（n = 6）不接受干预。通过光学显微镜在每只大鼠的尿道和肛管的福尔马林固定、石蜡包埋组织块的五个非连续切片（5微米厚）中随机选择的五个视野中计数黏膜下血管，用苏木精-伊红染色。结果以每个高倍视野（×400）的平均血管数表示。
卵巢切除术使尿道和肛管黏膜下血管计数显著低于假手术组值（分别为7.41±0.98对5.46±0.82，P = 0.003；7.16±1.11对4.92±0.65，P = 0.0009）。雌激素使尿道血管计数恢复（7.76±0.88，P = 0.5），胃饥饿素或雌激素与胃饥饿素联合给药使血管计数显著增加至高于假手术组计数（分别为8.68±0.99，P = 0.04；9.72±1.21，P = 0.004）。雌激素、胃饥饿素以及雌激素与胃饥饿素联合给药也使肛管血管计数恢复到假手术组水平（分别为7.26±0.97，P = 0.8；6.56±0.78，P = 0.3；7.76±0.88，P = 0.3）。
雌激素和胃饥饿素联合或单独替代通过逆转卵巢切除所致中年大鼠尿道和肛管黏膜下血管数量减少产生有益作用。
