器官培养的角膜缘组织的上皮增殖潜能；对同种异体角膜缘移植和眼库的意义。
Epithelial proliferative potential of organ cultured corneoscleral rims; implications for allo-limbal transplantation and eye banking.
作者信息
Shanmuganathan V A, Rotchford A P, Tullo A B, Joseph A, Zambrano I, Dua H S
机构信息
Division of Ophthalmology and Visual Sciences, University Hospital, Nottingham NG7 2UH, UK.
出版信息
Br J Ophthalmol. 2006 Jan;90(1):55-8. doi: 10.1136/bjo.2005.071910.
AIMS
To determine the epithelial proliferative capacity of organ cultured limbal tissue and correlate this with various donor and eye banking factors.
METHODS
24 corneoscleral limbal (CSL) rims left over from penetrating keratoplasty were split in half and set up as in vitro explant cultures. Corneal epithelial proliferative potential (CEPP) was assessed by the number of "cycles" of growth achieved before explants underwent exhaustion and failure to generate an epithelium to subconfluence. The dependence of CEPP on the age of the donor, time of death to enucleation, time of enucleation to organ culture, and time in organ culture in the eye bank was determined.
RESULTS
CSL rims were capable of up to four cycles of culture with a wide variation between tissue samples. Of the various factors examined, death to enucleation time was the only statistically significant factor affecting the CEPP (regression coefficient: -0.062 (cycles/hour), CI -0.119 to -0.004, p = 0.037). Time in organ culture had little effect on CEPP.
CONCLUSIONS
Preselected organ cultured CSL rims from eye banks may offer a viable alternative tissue source for use in allo-limbal transplantation.
目的
确定器官培养的角膜缘组织的上皮增殖能力，并将其与各种供体和眼库因素相关联。
方法
将穿透性角膜移植术后剩余的24个角膜缘（CSL）环切成两半，建立体外植块培养。通过外植体耗尽并无法产生上皮至汇合状态之前实现的生长“周期”数来评估角膜上皮增殖潜能（CEPP）。确定CEPP对供体年龄、死亡至摘除眼球时间、摘除眼球至器官培养时间以及在眼库中器官培养时间的依赖性。
结果
CSL环能够进行多达四个培养周期，组织样本之间差异很大。在所检查的各种因素中，死亡至摘除眼球时间是唯一影响CEPP的具有统计学意义的因素（回归系数：-0.062（周期/小时），CI -0.119至-0.004，p = 0.037）。器官培养时间对CEPP影响很小。
结论
从眼库中预先选择的器官培养的CSL环可能为同种异体角膜缘移植提供一种可行的替代组织来源。
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