Barouch Dan H
Research East Room 113, Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
J Pathol. 2006 Jan;208(2):283-9. doi: 10.1002/path.1874.
Vaccine development has traditionally been an empirical discipline. Classical vaccine strategies include the development of attenuated organisms, whole killed organisms, and protein subunits, followed by empirical optimization and iterative improvements. While these strategies have been remarkably successful for a wide variety of viruses and bacteria, these approaches have proven more limited for pathogens that require cellular immune responses for their control. In this review, current strategies to develop and optimize gene-based vaccines are described, with an emphasis on novel approaches to improve plasmid DNA vaccines and recombinant adenovirus vector-based vaccines.
疫苗研发传统上是一门经验性学科。经典的疫苗策略包括减毒生物体、全灭活生物体和蛋白质亚单位的研发，随后进行经验性优化和迭代改进。虽然这些策略在针对多种病毒和细菌方面取得了显著成功，但事实证明，对于那些需要细胞免疫反应来控制的病原体，这些方法的局限性更大。在这篇综述中，描述了目前开发和优化基因疫苗的策略，重点是改进质粒DNA疫苗和基于重组腺病毒载体疫苗的新方法。
