基于变性高效液相色谱法的斯里兰卡FUT2基因分型及遗传变异分析
Denaturing high-performance liquid chromatography-based genotyping and genetic variation of FUT2 in Sri Lanka.
作者信息
Soejima Mikiko, Koda Yoshiro
机构信息
Department of Forensic Medicine and Human Genetics, Kurume University School of Medicine, Kurume, Japan.
出版信息
Transfusion. 2005 Dec;45(12):1934-9. doi: 10.1111/j.1537-2995.2005.00651.x.
BACKGROUND
Human ABO-secretor locus (FUT2) is highly polymorphic in many human populations.
STUDY DESIGN AND METHODS
The applicability of denaturing high-performance liquid chromatography (DHPLC) analysis was evaluated for genotyping the FUT2 in two Sri Lankan populations (Tamil and Sinhalese).
RESULTS
Although DHPLC failed to detect one allele, 302C>T, of eight alleles, this method reduces the number of samples to be sequenced and can detect novel polymorphisms by comparing the elution profiles.
CONCLUSION
These results suggest that DHPLC analysis is a useful high-throughput method for genotyping FUT2 and, further, that the genetic backgrounds of two Sri Lankan populations are quite similar, with little genetic flow from neighboring East and Southeast Asian populations to Sri Lanka.
背景
人类ABO分泌型基因座（FUT2）在许多人群中具有高度多态性。
研究设计与方法
评估了变性高效液相色谱（DHPLC）分析在两个斯里兰卡人群（泰米尔族和僧伽罗族）中对FUT2进行基因分型的适用性。
结果
尽管DHPLC未能检测到八个等位基因中的一个等位基因302C>T，但该方法减少了需测序的样本数量，并且通过比较洗脱图谱能够检测到新的多态性。
结论
这些结果表明，DHPLC分析是一种用于FUT2基因分型的有用的高通量方法，此外，两个斯里兰卡人群的遗传背景非常相似，来自邻近东亚和东南亚人群向斯里兰卡的基因流动很少。
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