Raji Yinusa, Oloyo Ahmed Kolade, Morakinyo Ayodele Olufemi
Department of Physiology, College of Medicine, University of Ibadan, Ibadan 23 402, Nigeria.
Asian J Androl. 2006 Jan;8(1):115-21. doi: 10.1111/j.1745-7262.2006.00055.x.
To investigate the effect of methanol extract of Ricinus communis seed (RCE) on male rats reproductive functions.
Thirty-two male albino rats were divided into four groups. Groups 1, 2 and 3 were gavaged with 0.2 mL of 2.5% tween 80 (RCE vehicle; control) or 20 mg/(kg x d) and 40 mg/(kg x d) of RCE, respectively, for 30 days, and group 4 was also gavaged with 40 mg/(kg x d) of RCE, but was allowed a recovery period of 30 days. Five untreated female rats were cohabited with male rats in each group from day 25 of RCE treatment for 5 days, except group 4, where cohabitation began on day 25 of the recovery period. All male rats were sacrificed 24 h after the experiments. The female rats were laparotomized on day 19 of pregnancy and the number and weight of litters were recorded.
There was a significant decrease (P<0.01) in the weight of the reproductive organs, sperm functions and serum levels of testosterone in RCE treated rats. There was disorganization in the cytoarchitecture of the testes, disruption of the seminiferous tubules and erosion of the germinal epithelium. The number and weight of litters of rats in groups 2 and 4 decreased significantly (P<0.05) but no changes were observed in group 3. RCE caused no changes in liver, kidney, heart or body weights in male rats.
RCE has a reversible negative impact on male reproductive functions, which appears to be mediated via gonadal disruption in testosterone secretion.
研究蓖麻籽甲醇提取物（RCE）对雄性大鼠生殖功能的影响。
将32只雄性白化大鼠分为四组。第1、2和3组分别用0.2 mL 2.5%吐温80（RCE赋形剂；对照组）或20 mg/（kg·d）和40 mg/（kg·d）的RCE灌胃30天，第4组也用40 mg/（kg·d）的RCE灌胃，但给予30天的恢复期。从RCE治疗第25天起，每组将5只未处理的雌性大鼠与雄性大鼠合笼5天，但第4组在恢复期第25天开始合笼。实验结束24小时后处死所有雄性大鼠。在妊娠第19天对雌性大鼠进行剖腹手术，并记录产仔数和仔鼠体重。
RCE处理的大鼠生殖器官重量、精子功能和血清睾酮水平显著降低（P<0.01）。睾丸细胞结构紊乱，生精小管破坏，生殖上皮糜烂。第2组和第4组大鼠的产仔数和仔鼠体重显著下降（P<0.05），但第3组未观察到变化。RCE对雄性大鼠的肝脏、肾脏、心脏或体重无影响。
RCE对雄性生殖功能有可逆的负面影响，这似乎是通过性腺干扰睾酮分泌介导的。
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