Human Anatomy and Embryology Department, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt.
Medical Biochemistry Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
Sci Rep. 2024 Jul 31;14(1):17743. doi: 10.1038/s41598-024-67636-z.
Pesticides like atrazine which are frequently present in everyday surroundings, have adverse impacts on human health and may contribute to male infertility. The work aimed to analyze the histological and biochemical effects of atrazine on the testis in adult albino rats and whether co-administration with resveratrol could reverse the effect of atrazine. Forty adult male albino rats in good health participated in this study. They were categorized at random into four groups: the Group Ӏ received water through a gastric tube for two months every day, the Group ӀӀ received resveratrol (20 mg/kg body weight (b.w.)) through a gastric tube for two months every day, the Group ӀӀӀ received atrazine (50 mg/kg bw) through a gastric tube for two months every day, the Group ӀV received concomitant doses of atrazine and resveratrol for two months every day. The testes of the animals were then carefully removed and prepared for biochemical, immunohistochemical, light, and electron microscopic studies. Atrazine exposure led to a significant decrease in serum testosterone hormone level, upregulation of caspase 3 and iNOS mRNA levels, destructed seminiferous tubules with few sperms in their lumens, many collagen fibres accumulation in the tunica albuginea and the interstitium, abnormal morphology of some sperms as well as many vacuolations, and damaged mitochondria in the cytoplasm of many germ cells. Concomitant administration of resveratrol can improve these adverse effects. It was concluded that atrazine exposure is toxic to the testis and impairs male fertility in adult rat and coadministration of resveratrol guards against this toxicity.
莠去津等农药在日常生活环境中经常存在，对人类健康有不良影响，并可能导致男性不育。本研究旨在分析莠去津对成年白化大鼠睾丸的组织学和生化影响，以及白藜芦醇是否能逆转莠去津的作用。40 只健康成年雄性白化大鼠参与了这项研究。它们被随机分为四组：第 Ӂ 组每天通过胃管给予水，第 ӁӁ 组每天通过胃管给予白藜芦醇（20mg/kg 体重），第 ӁӁӁ 组每天通过胃管给予莠去津（50mg/kg bw），第 ӁV 组每天同时给予莠去津和白藜芦醇。然后小心取出动物的睾丸，准备进行生化、免疫组织化学、光镜和电镜研究。莠去津暴露导致血清睾酮激素水平显著下降，caspase 3 和 iNOS mRNA 水平上调，生精小管破坏，管腔中精子很少，白膜和间质中胶原纤维大量堆积，一些精子形态异常，许多精子细胞质中线粒体空泡化和损伤。同时给予白藜芦醇可以改善这些不良反应。结论是，莠去津暴露对睾丸有毒性，损害成年大鼠的生育能力，而白藜芦醇的共同给药可预防这种毒性。
