Whitehead Geoffrey G, Makino Shinji, Lien Ching-Ling, Keating Mark T
Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Department of Cardiology, Children's Hospital, Boston, MA 02115, USA.
Science. 2005 Dec 23;310(5756):1957-60. doi: 10.1126/science.1117637.
Epimorphic regeneration requires the presence or creation of pluripotent cells capable of reproducing lost organs. Zebrafish fin regeneration is mediated by the creation of blastema cells. Here, we characterize the devoid of blastema (dob) mutant that fails fin regeneration during initial steps, forms abnormal regeneration epithelium, and does not form blastema. This mutation has no impact on embryonic survival. Dob results from an fgf20a null mutation, Y148S. Fgf20a is expressed during initiation of fin regeneration at the epithelial-mesenchymal boundary and later overlaps with the blastema marker msxb. Thus, fgf20a has a regeneration-specific requirement, initiating fin regeneration, and controlling blastema formation.
形态发生再生需要存在或产生能够再生缺失器官的多能细胞。斑马鱼鳍的再生是由胚基细胞的产生介导的。在这里，我们对缺乏胚基（dob）的突变体进行了表征，该突变体在初始阶段无法进行鳍再生，形成异常的再生上皮，并且不形成胚基。这种突变对胚胎存活没有影响。Dob是由fgf20a的无效突变Y148S导致的。Fgf20a在鳍再生起始阶段在上皮-间充质边界表达，随后与胚基标记物msxb重叠。因此，fgf20a对再生有特定需求，启动鳍再生并控制胚基形成。
