Ortega María Florencia, Bonamy Martín, Cutullé Christian, Giovambattista Guillermo
Estación Experimental Agropecuaria Famaillá (EEA Famaillá), Agencia de Extensión Rural Lules (AER Lules), Instituto Nacional de Tecnología Agropecuaria (INTA), Lules, Tucumán, 4129, Argentina.
Facultad de Ciencias Veterinarias, Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout" (IGEVET, UNLP-CONICET LA PLATA), Universidad Nacional de La Plata, La Plata (B1904), Buenos Aires, Argentina.
Trop Anim Health Prod. 2024 Sep 27;56(8):289. doi: 10.1007/s11250-024-04110-y.
The common cattle tick Rhipicephalus microplus causes severe limitations to livestock production. Bovine genetics could be a decisive component for the success or failure of control programs for ticks and diseases transmitted. The objective of this work was to detect chromosomal regions associated with host resistance to R. microplus through an associative mapping study using medium and high density microarrays in a population of Argentine Creole cattle. The phenotypic record of the number of ticks that completed their development on the host, after artificial infestations, was obtained during 2015 to 2020. Genomic DNA was extracted for genotyping from 192 animals using Affymetrix high (Axiom™ Bos 1) and medium density (ArBos1) microarrays. In an exploratory study, chromosomal regions containing putative quantitative trait loci (QTLs) were recognized on chromosomes 27, 11, 10, 9, 16, 13, 3, 19, 8 and 18, associated with the variation of R. microplus load. Gene ontology based on genes located on these regions revealed an enrichment of terms and pathways for the immune system, blood coagulation, tissue regeneration, endopeptidase activity and protein phosphorylation. The information obtained in this work constitutes a first report of QTLs for tick count in the Argentine Creole cattle, and contributes with the knowledge about the underlying process involved in tick resistance.
常见的牛蜱微小扇头蜱对牲畜生产造成严重限制。牛的遗传学可能是蜱虫及所传播疾病控制计划成败的决定性因素。本研究的目的是通过在一群阿根廷克里奥尔牛中使用中高密度微阵列进行关联图谱研究，检测与宿主对微小扇头蜱抗性相关的染色体区域。在2015年至2020年期间，记录了人工感染后在宿主身上完成发育的蜱虫数量的表型数据。使用Affymetrix高密度（Axiom™ Bos 1）和中密度（ArBos1）微阵列从192只动物中提取基因组DNA进行基因分型。在一项探索性研究中，在27号、11号、10号、9号、16号、13号、3号、19号、8号和18号染色体上识别出了包含假定数量性状位点（QTL）的染色体区域，这些区域与微小扇头蜱负载量的变化有关。基于这些区域上的基因进行的基因本体分析显示，免疫系统、血液凝固、组织再生、内肽酶活性和蛋白质磷酸化等术语和途径得到了富集。本研究获得的信息构成了阿根廷克里奥尔牛蜱虫计数QTL的首次报告，并有助于了解蜱虫抗性所涉及的潜在过程。
