Rojas-Dotor Sara, Rico Guadalupe, Pérez Julia, Velázquez Juan, Silva Raúl, Morales Esther, Kretschmer Roberto
Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI (CMN-SXXI), Instituto Mexicano del Seguro Social, Mexico City, DF, Mexico.
Parasitol Res. 2006 Apr;98(5):493-5. doi: 10.1007/s00436-005-0090-y. Epub 2005 Dec 22.
Entamoeba histolytica produces monocyte locomotion inhibitory factor (MLIF), a pentapeptide with in vitro and in vivo anti-inflammatory properties. MLIF may interfere with leukocyte migration, disturbing the balance of pro- and anti-inflammatory cytokines secreted by CD4(+) T lymphocytes. We evaluated the effect of MLIF on expression of pro- and anti-inflammatory cytokines in human CD4(+) T lymphocytes. Regulatory cytokines [interleukin-1 beta (IL-1beta), IL-2, interferon gamma (IFN-gamma), IL-5, IL-6, and IL-10] were studied by enzyme-linked immunosorbent assay method in CD4(+)-cell supernatant fluids. Proinflammatory cytokines were produced per se by MLIF (IL-1beta, IL-2, and IFN-gamma) and also anti-inflammatory cytokines (IL-5, IL-6, and IL-10) with 1-phorbol-12 myristate-13 acetate + MLIF; the IL-1beta, IFN-gamma, IL-5 and IL-6 production was inhibited but not that of IL-10 which disclosed increase in its expression. MLIF disturbs the pro- and anti-inflammatory balance, and it induces inhibition of IL-1beta (principal proinflammatory cytokine) and increases IL-10 (prototype of an anti-inflammatory cytokine).
溶组织内阿米巴产生单核细胞移动抑制因子（MLIF），这是一种具有体外和体内抗炎特性的五肽。MLIF可能会干扰白细胞迁移，扰乱CD4(+) T淋巴细胞分泌的促炎和抗炎细胞因子的平衡。我们评估了MLIF对人CD4(+) T淋巴细胞中促炎和抗炎细胞因子表达的影响。通过酶联免疫吸附测定法在CD4(+)细胞上清液中研究调节性细胞因子[白细胞介素-1β（IL-1β）、IL-2、干扰素γ（IFN-γ）、IL-5、IL-6和IL-10]。MLIF本身可产生促炎细胞因子（IL-1β、IL-2和IFN-γ），1-佛波醇-12肉豆蔻酸酯-13乙酸酯+MLIF也可产生抗炎细胞因子（IL-5、IL-6和IL-10）；IL-1β、IFN-γ、IL-5和IL-6的产生受到抑制，但IL-10的产生未受抑制，其表达反而增加。MLIF扰乱了促炎和抗炎平衡，它诱导抑制IL-1β（主要促炎细胞因子）并增加IL-10（抗炎细胞因子的原型）。
