Massart Catherine, Gibassier Jacqueline, Laurent Marie-Christine, Le Lannou Dominique Le
Laboratoire de Génétique Moléculaire et Hormonologie, CHU de Pontchaillou, Rennes, France.
Clin Chem Lab Med. 2006;44(1):105-9. doi: 10.1515/CCLM.2006.020.
Measurement of estradiol is useful in women undergoing ovarian stimulation for in vitro fertilization and embryo transfer (IVF-ET). The analytical performance of a new two-step estradiol assay (ADVIA Centaur) estradiol-6 III from Bayer Diagnostics) was evaluated in 41 sera from 11 women undergoing ovarian stimulation. The results were compared to those obtained with two radioimmunoassays (RIAs; RIA Estradiol Immunotech IM 1663 from Beckmann Coulter and Coat-A-Count Estradiol from Diagnostic Products Corporation) and with one chemiluminescent immunoassay (CLIA; ADVIA Centaur estradiol-6). The ADVIA Centaur) estradiol-6 III assay was the most sensitive assay, with a functional sensitivity of 55 pmol/L. Within- and between-run coefficients of variation calculated for the new ADVIA Centaur assay ranged from 3.3% to 9%, which was better than the precision obtained for the other assays. A dilution test showed serum interferences when estradiol was measured in non-diluted samples. No statistical difference was observed between the estradiol results obtained in diluted sera with the new two-step ADVIA Centaur assay and those measured with the Immunotech RIA and the other CLIA. In conclusion, this new, two-step estradiol assay performed on the ADVIA Centaur system displays suitable sensitivity, precision and intermethod agreement with the Immunotech RIA for the measurement of serum estradiol concentrations in women undergoing ovarian stimulation and IVF-ET. For correct linearity, estradiol measurement should be performed on diluted samples.
对于接受体外受精和胚胎移植（IVF-ET）卵巢刺激的女性，雌二醇测量很有用。对来自11名接受卵巢刺激女性的41份血清，评估了一种新的两步法雌二醇检测（ADVIA Centaur系统的雌二醇-6 III，拜耳诊断公司产品）的分析性能。将结果与两种放射免疫测定法（RIA；贝克曼库尔特公司的RIA Estradiol Immunotech IM 1663和诊断产品公司的Coat-A-Count Estradiol）以及一种化学发光免疫测定法（CLIA；ADVIA Centaur系统的雌二醇-6）所得结果进行比较。ADVIA Centaur系统的雌二醇-6 III检测是最灵敏的检测方法，功能灵敏度为55 pmol/L。为新的ADVIA Centaur检测计算的批内和批间变异系数在3.3%至9%之间，优于其他检测方法的精密度。稀释试验表明，在未稀释样本中测量雌二醇时存在血清干扰。在稀释血清中用新的两步法ADVIA Centaur检测获得的雌二醇结果与用Immunotech RIA和另一种CLIA测量的结果之间未观察到统计学差异。总之，在ADVIA Centaur系统上进行的这种新的两步法雌二醇检测在测量接受卵巢刺激和IVF-ET女性的血清雌二醇浓度时，显示出合适的灵敏度、精密度以及与Immunotech RIA的方法间一致性。为获得正确的线性，应在稀释样本上进行雌二醇测量。
