Seo Sang-Soo, Song Yong-Sang, Kim Jae-Weon, Park Noh-Hyun, Kang Soon-Beom, Lee Hyo-Pyo
Center for Uterine Cancer, National Cancer Center, 809 Madu1-dong, Goyang-si, Gyeonggi-do 411-351, Korea.
Gynecol Oncol. 2006 Jul;102(1):67-73. doi: 10.1016/j.ygyno.2005.11.030. Epub 2006 Jan 10.
To evaluate the efficacy of self-collected vaginal samples for high-risk HPV detection by the HPV oligonucleotide microarray method (HPVDNAChip).
One hundred and eighteen patients with abnormal Pap smears were included. Self-collected vaginal and clinician-collected cervical samples for HPV testing were obtained. The result of the HPV DNA test was compared with the histopathological diagnosis or colposcopic finding.
Of the 118 patients, 42 (35.6%) had >or= cervical intraepithelial neoplasia (CIN) III lesions. Using the HPVDNAChip, high-risk types of HPV were detected in 38 of these 42 patients (90.5%) with the self-collected vaginal samples and in 37 of 42 (88.1%) with the clinician-collected cervical samples. The agreement of HPVDNAchip results between self- and clinician-collected samples was very good (kappa = 0.81) with a 93.2% concordance rate. Multiple HPV infections were found in 17 of 88 (19.3%) HPV-positive clinician-collected cervical samples. The rate of multiple HPV infection tended to decrease as the degree of pathologic classification increased.
Using the HPVDNAchip to assay for HPV infection, results from self-collected vaginal samples were compatible with those from clinician-collected cervical samples.
通过人乳头瘤病毒寡核苷酸微阵列法（HPV DNA芯片）评估自行采集的阴道样本用于高危型HPV检测的效果。
纳入118例巴氏涂片异常的患者。获取自行采集的阴道样本和临床医生采集的宫颈样本进行HPV检测。将HPV DNA检测结果与组织病理学诊断或阴道镜检查结果进行比较。
118例患者中，42例（35.6%）患有≥宫颈上皮内瘤变（CIN）III级病变。使用HPV DNA芯片，在这42例患者中，38例（90.5%）通过自行采集的阴道样本检测到高危型HPV，42例中的37例（88.1%）通过临床医生采集的宫颈样本检测到高危型HPV。自行采集样本与临床医生采集样本的HPV DNA芯片检测结果一致性非常好（kappa = 0.81），一致率为93.2%。在88例HPV阳性的临床医生采集的宫颈样本中，17例（19.3%）发现多重HPV感染。随着病理分级程度增加，多重HPV感染率呈下降趋势。
使用HPV DNA芯片检测HPV感染时，自行采集的阴道样本结果与临床医生采集的宫颈样本结果相符。
