Simões Claudia, De Mattos José Carlos P, Sabino Kátia C C, Caldeira-de-Araújo Adriano, Coelho Marsen G P, Albarello Norma, Figueiredo Solange F L
Departamento de Biologia Vegetal/Laboratório de Biotecnologia de Plantas (LABPLAN), Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Brazil.
Fitoterapia. 2006 Feb;77(2):94-9. doi: 10.1016/j.fitote.2005.11.001. Epub 2006 Jan 10.
Methanolic extracts obtained from different organs of Cleome rosea, collected from its natural habitat and from in vitro-propagated plants, were submitted to in vitro biological assays. Inhibition of nitric oxide (NO) production by J774 macrophages and antioxidant effects by protecting the plasmid DNA from the SnCl(2)-induced damage were evaluated. Extracts from the stem of both origins and leaf of natural plants inhibited NO production. The plasmid DNA strand breaks induced by SnCl(2) were reduced by extracts from either leaf or stem of both sources. On the other hand, root extracts did not show any kind of effects on plasmid DNA, and presented significant toxic effects to J774 cells. The results showed that C. rosea presents medicinal potential and that the acclimatization process reduces the plant toxicity both to plasmid DNA and to J774 cells, suggesting the use of biotechnology tools to obtain elite plants as source of botanical material for pharmacological and phytochemical studies.
从玫瑰醉蝶花自然生长地和体外繁殖植株的不同器官中提取的甲醇提取物，被用于体外生物学测定。评估了其对J774巨噬细胞产生一氧化氮（NO）的抑制作用以及通过保护质粒DNA免受氯化亚锡（SnCl₂）诱导损伤的抗氧化作用。来自两种来源的茎提取物和天然植物的叶提取物均抑制了NO的产生。两种来源的叶或茎提取物均减少了由SnCl₂诱导的质粒DNA链断裂。另一方面，根提取物对质粒DNA未显示任何作用，且对J774细胞呈现显著毒性作用。结果表明，玫瑰醉蝶花具有药用潜力，并且驯化过程降低了该植物对质粒DNA和J774细胞的毒性，这表明可利用生物技术工具来获得优良植株，作为药理学和植物化学研究的植物材料来源。
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