Ferreira-Machado S C, Rodrigues M P, Nunes A P M, Dantas F J S, De Mattos J C P, Silva C R, Moura E G, Bezerra R J A C, Caldeira-de-Araujo A
Departamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Av. 28 de setembro, 87, Rio de Janeiro 20551-030, Brazil.
Toxicol Lett. 2004 Aug 1;151(3):481-7. doi: 10.1016/j.toxlet.2004.03.014.
Plants have been related to our lives, being used as medicine, regardless of scientific evidence of side effects. This work analyses the toxicological effects of Chrysobalanus icaco L. aqueous extract, used in different pathologies. It was studied through: (i) alteration of plasmid pUC 9.1 topology; (ii) survival of bacterial strains submitted, or not, to previous treatment with SnCl2; (iii) transformation efficiency of E. coli strain by the treatment with the plasmid pUC 9.1. In (i), the treatment of the plasmid resulted in DNA single-strand breaks (SSB). A decrease of the lethal effect induced by SnCl2 in presence of the extract was found, while no C. icaco bacterial survival reduction was observed. The transformation efficiency of the plasmid was also reduced. Results suggest that the extract could present a potential genotoxic effect, as demonstrated either by the induction of SSB in plasmid or in transformation efficiency experiments. Finally, it presents an antioxidant action.
植物与我们的生活息息相关，被用作药物，而不顾其副作用的科学证据。这项工作分析了用于不同病症的可可李（Chrysobalanus icaco L.）水提取物的毒理学效应。通过以下方面进行了研究：（i）质粒pUC 9.1拓扑结构的改变；（ii）接受或未接受先前SnCl2处理的细菌菌株的存活率；（iii）用质粒pUC 9.1处理对大肠杆菌菌株的转化效率。在（i）中，质粒处理导致DNA单链断裂（SSB）。发现在提取物存在的情况下，SnCl2诱导的致死效应降低，而未观察到可可李细菌存活率降低。质粒的转化效率也降低了。结果表明，该提取物可能具有潜在的遗传毒性作用，这在质粒中的SSB诱导或转化效率实验中均有体现。最后，它具有抗氧化作用。
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