Naoumidi Tatiana L, Pallikaris Ioannis G, Naoumidi Irini I, Astyrakakis Nikolaos I
University of Crete, School of Medicine, Vardinoyannion Eye Institute of Crete, Voutes PO 1352, 71110 Heraklion, Crete, Greece.
Am J Ophthalmol. 2005 Dec;140(6):984-992. doi: 10.1016/j.ajo.2005.06.027.
To determine the morphologic changes in human corneas over time following radiofrequency-based conductive keratoplasty (CK) treatment.
Prospective, observational case series.
In a single-center study six human corneas of six patients with localized peripheral keratoconus underwent CK treatment followed by penetrating keratoplasty. Three spots were applied in the periphery of each cornea (6 mm optical zone). Corneal buttons were examined with light and electron microscopy at different postoperative intervals up to 6 months post-CK.
In samples assessed on day one post-CK, small areas of detachment between the basal layer of epithelial cells and Bowman's layer were observed. At 1 week after the CK procedure, the epithelium appeared almost normal. Endothelium and Descemet's membrane had no alterations. In all samples, thermally induced misconfiguration of collagen fibers, described as "crumpled" changes of collagen layers, was observed reaching 75% to 80% of the stromal depth. The area of alterations had a cylindrical shape with a diameter of 120 mum.
The conductive keratoplasty procedure produced collagen "crumpling and splitting" changes in human corneas, which were observed during the follow-up of 6 months. Areas adjacent to treatment site were minimally damaged.
确定基于射频的传导性角膜成形术（CK）治疗后人类角膜随时间的形态学变化。
前瞻性观察病例系列。
在一项单中心研究中，对6例局限性周边圆锥角膜患者的6只人眼角膜进行CK治疗，随后行穿透性角膜移植术。在每个角膜周边（6mm光学区）施加3个点。在CK术后不同时间间隔直至6个月，用光镜和电镜检查角膜片。
在CK术后第1天评估的样本中，观察到上皮细胞基底层与Bowman层之间有小面积分离。CK术后1周，上皮几乎恢复正常。内皮和Descemet膜无改变。在所有样本中，均观察到热诱导的胶原纤维构型异常，表现为胶原层的“皱缩”变化，达基质深度的75%至80%。改变区域呈圆柱形，直径为120μm。
传导性角膜成形术在人眼角膜中产生了胶原“皱缩和分裂”变化，在6个月的随访中观察到。治疗部位附近区域受损最小。
Zotero 插件