Breuillin Florence, Dutech Cyril, Robin Cécile
Institut National de la Recherche Agronomique, Unité Mixte de Recherche BIOGECO, Equipe de Pathologie Forestière, Domaine de la Grande Ferrade, 71 Villenave d'Ornon, France.
Mycol Res. 2006 Mar;110(Pt 3):288-96. doi: 10.1016/j.mycres.2005.09.016. Epub 2005 Dec 27.
Microsatellites are powerful markers to infer population genetic parameters. Here, 13 microsatellite loci isolated from a genomic and a cDNA library of Cryphonectria parasitica were used to characterize the genetic diversity and structure of four French populations. Twelve of these loci were polymorphic within populations, and average gene diversity (H(e)) was estimated to be 0.35. There was a lower genetic diversity in a south-eastern population relative to three south-western populations. In these three populations, microsatellite genotypic diversity was higher than vegetative compatibility type diversity. A high genetic differentiation (G(ST) = 0.27) suggested a low gene flow and/or founder effects of French populations which are in agreement with low dispersal of spores and different introductions of this species in southern France. This study demonstrates the significance of these microsatellite loci to assess gene flow and reproductive system in this important pathogen.
微卫星是推断种群遗传参数的有力标记。在此，从寄生隐孢菌的基因组文库和cDNA文库中分离出的13个微卫星位点被用于表征四个法国种群的遗传多样性和结构。这些位点中有12个在种群内具有多态性，平均基因多样性（H(e)）估计为0.35。相对于三个西南部种群，东南部种群的遗传多样性较低。在这三个种群中，微卫星基因型多样性高于营养体亲和性类型多样性。较高的遗传分化（G(ST) = 0.27）表明法国种群的基因流较低和/或存在奠基者效应，这与孢子的低扩散以及该物种在法国南部的不同引入情况相符。本研究证明了这些微卫星位点在评估这种重要病原体的基因流和生殖系统方面的重要性。
