[通过HLA-A2限制性NY-ESO-1b肽体外诱导的NY-ESO-1b特异性CD8+T细胞对表达NY-ESO-1的肝癌细胞系进行评估]

[Estimation of an NY-ESO-1 expressing HCC cell line by NY-ESO-1b specific CD8+T cells in vitro induced by HLA-A2 restricted NY-ESO-1b peptide].

作者信息

Qiao Huan, Qian Xiao-ping, Zhang Hua-gang, Tian Chan, Chen Wei-feng

机构信息

T Cell Laboratory of Department of Immunology, Peking University School of Basic Medical Science, Beijing 100083, China.

出版信息

Beijing Da Xue Xue Bao Yi Xue Ban. 2005 Dec 18;37(6):565-8.

PMID:16378102

Abstract

OBJECTIVE

To analyze the effect of an NY-ESO-1 expressing HCC cell line by NY-ESO-1b specific CD8(+)T cells in vitro induced by HLA-A2 Restricted NY-ESO-1b peptide and to further analyze the possibility of NYjESOj1 as a vaccine in HCC treatment.

METHODS

Peripheral blood mononuclear cells (PBMCs) were obtained from an HLA-A2(+) HCC patient and NY-ESO-1 specific CD8(+)T cells were in vitro induced by HLA-A2 Restricted NY-ESO-1b peptide. Next we estimated the effect that NY-ESO-1 specific CD8(+)T cells recognized and killed NY-ESO-1 expressing HepG2 cells by enzyme-linked immunospot(ELISPOT).

RESULTS

These in vitro induced NY-ESO-1b specific CD8(+)T cells could recognize HepG2 cells stably transfected with NY-ESO-1 in both IFN-gamma and Granzyme B ELISPOT assays. And the data also showed that NY-ESO-1 specific CD8(+)T cells could efficiently kill NY-ESO-1 expressing HepG2 cells.

CONCLUSION

NY-ESO-1 protein can be processed by HCC cells, and that HLA-A2 restricted NY-ESO-1b peptide (157-165) can also be presented on the surfaces of cells and recognized by NY-ESO-1b specific CD8(+)T cells in vitro induced.

摘要

目的

分析由 HLA - A2 限制性 NY - ESO - 1b 肽体外诱导的 NY - ESO - 1b 特异性 CD8(+)T 细胞对表达 NY - ESO - 1 的肝癌细胞系的作用，并进一步分析 NY - ESO - 1 作为肝癌治疗疫苗的可能性。

方法

从一名 HLA - A2(+)肝癌患者获取外周血单个核细胞（PBMC），并用 HLA - A2 限制性 NY - ESO - 1b 肽体外诱导 NY - ESO - 1 特异性 CD8(+)T 细胞。接下来，我们通过酶联免疫斑点法（ELISPOT）评估 NY - ESO - 1 特异性 CD8(+)T 细胞识别并杀伤表达 NY - ESO - 1 的 HepG2 细胞的效果。

结果

在 IFN -γ 和颗粒酶 B 的 ELISPOT 检测中，这些体外诱导的 NY - ESO - 1b 特异性 CD8(+)T 细胞能够识别稳定转染 NY - ESO - 1 的 HepG2 细胞。数据还表明，NY - ESO - 1 特异性 CD8(+)T 细胞能够有效杀伤表达 NY - ESO - 1 的 HepG2 细胞。