Aung Cho S, Faddy Helen M, Lister Erin J, Monteith Gregory R, Roberts-Thomson Sarah J
The School of Pharmacy, The University of Queensland, Brisbane, Qld 4072, Australia.
Biochem Biophys Res Commun. 2006 Feb 10;340(2):656-60. doi: 10.1016/j.bbrc.2005.12.061. Epub 2005 Dec 20.
To investigate the role of peroxisome proliferator-activated receptors (PPARs) alpha and beta in the differentiation of colon cancer cells, we differentiated HT-29 cells using sodium butyrate (NaB) and culturing post-confluence and assessed differentiation using the marker intestinal alkaline phosphatase. While PPARalpha levels only changed with culturing post confluence, PPARbeta levels increased independent of the method of differentiation. To explore further the differences induced by NaB, we assessed changes in both PPAR isoforms in MCF-7 breast cancer cells cultured in the presence of NaB over 48h. Again a very different expression pattern was observed with PPARalpha increasing after 4h and remaining elevated, while PPARbeta increased transiently. Our studies suggest that the expression of PPARs is dependent upon both the method of differentiation and on time. Moreover, these studies show that changes in PPARalpha levels are not required for the differentiation of colon cancer cell lines, whereas changes in PPARbeta are more closely associated with differentiation.
为了研究过氧化物酶体增殖物激活受体（PPARs）α和β在结肠癌细胞分化中的作用，我们使用丁酸钠（NaB）对HT-29细胞进行分化处理，并在汇合后进行培养，然后使用标志物肠碱性磷酸酶评估分化情况。虽然PPARα水平仅在汇合后培养时发生变化，但PPARβ水平的升高与分化方法无关。为了进一步探究NaB诱导的差异，我们评估了在NaB存在下培养48小时的MCF-7乳腺癌细胞中两种PPAR亚型的变化。同样观察到了非常不同的表达模式，PPARα在4小时后增加并持续升高，而PPARβ则短暂增加。我们的研究表明，PPARs的表达既取决于分化方法，也取决于时间。此外，这些研究表明，结肠癌细胞系的分化不需要PPARα水平的变化，而PPARβ的变化与分化更密切相关。
