Pellicano Francesca, Inglis-Broadgate Suzanne L, Pante Guido, Ansorge Wilhelm, Iwata Tomoko
Beatson Laboratories for Cancer Research, Division of Cancer Sciences and Molecular Pathology, Faculty of Medicine, University of Glasgow, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Gene Expr Patterns. 2006 Mar;6(3):285-93. doi: 10.1016/j.modgep.2005.07.004. Epub 2005 Dec 27.
Fibroblast growth factor 2 (FGF2) plays an important role in cortical development. However, the genes downstream of FGF2 that mediate its effect are largely unknown. We have performed a microarray screening of genes regulated by FGF2 using primary cortical neuron culture derived from embryonic day 14.5 (E14.5) mouse forebrains. In this study, we have analysed a previously uncharacterised gene encoding a 180-amino acid protein, hereby named 'coiled-coil protein 1 (ccp1)', that showed a modest up-regulation upon FGF2 stimulation. Northern blots and RT-PCR showed specific expression of ccp1 in multiple tissues including adult and embryonic brains. In situ hybridizations revealed that ccp1 was expressed in the cortical plate between Reelin and Tbr1-positive layers in the dorsal cortex at E15.5. Furthermore, the expression pattern of ccp1 at E13.5-E14.5 reflected some of the aspects of tangential migration of cortical progenitors during the early phase. We observed that the expressed ccp1 protein was localised to endo/lysosomal compartment in the cell body as well as to vesicles present in the processes of primary cortical neurons and oligodendrocyte cell line.
成纤维细胞生长因子2（FGF2）在皮质发育中起重要作用。然而，介导其作用的FGF2下游基因在很大程度上尚不清楚。我们使用源自胚胎第14.5天（E14.5）小鼠前脑的原代皮质神经元培养物对受FGF2调控的基因进行了微阵列筛选。在本研究中，我们分析了一个以前未表征的基因，该基因编码一种180个氨基酸的蛋白质，在此命名为“卷曲螺旋蛋白1（ccp1）”，其在FGF2刺激后表现出适度上调。Northern印迹和RT-PCR显示ccp1在包括成年和胚胎脑在内的多种组织中特异性表达。原位杂交显示，在E15.5时，ccp1在背侧皮质中Reelin和Tbr1阳性层之间的皮质板中表达。此外，ccp1在E13.5 - E14.5的表达模式反映了早期皮质祖细胞切向迁移的一些方面。我们观察到，表达的ccp1蛋白定位于细胞体中的内体/溶酶体区室以及原代皮质神经元和少突胶质细胞系突起中的小泡。
