Moriyama Y, Goto T, Hashimoto S, Furukawa T, Kishi K, Takahashi M, Shibata A
First Department of Internal Medicine, Niigata University School of Medicine, Japan.
Ann Hematol. 1992 Jun;64(6):266-9. doi: 10.1007/BF01695468.
The application of hyperthermia to the treatment of neoplastic disease has focused on solid tumors. Since the hyperthermic sensitivity of human B-cell lymphoma cells is not known, we have examined the effect of hyperthermia on the growth of B-cell lymphoma cell lines (Raji and Daudi) in vitro to evaluate the ability to purge tumor cells from normal bone marrow by heat, utilizing a limiting-dilution assay to measure log depletion of tumor cells in a 20-fold excess of normal bone marrow. When exposed at 42 degrees C and 43 degrees C for 120 min, both clonogenic Raji and Daudi cells were dramatically decreased (a 4- to 6-log reduction) with exposure time, while leaving over half of the normal granulocyte-macrophage progenitor cells surviving at 42 degrees C and 10% at 43 degrees C. This high level of lymphoma-cell depletion by heat correlated with that obtained in immunologic and pharmacologic studies. These results suggest that in vitro hyperthermia might be applied effectively for the elimination of residual lymphoma cells in autologous marrow grafts before autologous bone marrow transplantation in B-cell non-Hodgkin's lymphoma.
热疗在肿瘤疾病治疗中的应用主要集中于实体瘤。由于人类B细胞淋巴瘤细胞的热敏感性尚不清楚，我们检测了热疗对B细胞淋巴瘤细胞系（拉吉细胞和道迪细胞）体外生长的影响，以评估通过加热从正常骨髓中清除肿瘤细胞的能力，采用极限稀释法来测量在20倍过量的正常骨髓中肿瘤细胞的对数减少情况。当在42℃和43℃下暴露120分钟时，克隆形成的拉吉细胞和道迪细胞均随暴露时间显著减少（减少4至6个对数级），而在42℃时超过一半的正常粒细胞-巨噬细胞祖细胞存活，在43℃时为10%。热疗导致的这种高水平淋巴瘤细胞清除与免疫和药理学研究结果相关。这些结果表明，在B细胞非霍奇金淋巴瘤患者进行自体骨髓移植前，体外热疗可能有效地用于清除自体骨髓移植物中残留的淋巴瘤细胞。
