Zhang Liu-Suo, Xiang Jian-Hai
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Yi Chuan. 2005 Nov;27(6):919-24.
Two full-sib families of Litopenaeus vannamei were used to study the inheritance of allelic variation at 10 microsatellite loci. Of the 20 genotypic ratios observed (10 loci x 2 crosses), 17 ratios conformed to Mendelian segregation. When null alleles were considered, one loci (TUMXLv8.220) confirmed Mendelian expectations in all families. While one loci (TUMXLv3.1) showed deviation from family 06. Three loci (TUMXLv5.66,TUMXLv7.74,TUMXLv8.224)were monomorphic in both controlled crosses; 3 loci were polymorphic and confirmed to Mendelian ratios in all families, can be used for parental analysis and population genetic studies. These results indicated the need to test the inheritance pattern for microsatellite markers in shrimp before using them for population genetic or kinship analysis.
利用凡纳滨对虾的两个全同胞家系研究了10个微卫星位点上等位基因变异的遗传情况。在观察到的20个基因型比例中(10个位点×2个杂交组合),有17个比例符合孟德尔分离定律。当考虑无效等位基因时,一个位点(TUMXLv8.220)在所有家系中均符合孟德尔预期。而一个位点(TUMXLv3.1)在06家系中表现出偏离。三个位点(TUMXLv5.66、TUMXLv7.74、TUMXLv8.224)在两个对照杂交组合中均为单态性;3个位点具有多态性且在所有家系中均符合孟德尔比例,可用于亲本分析和群体遗传学研究。这些结果表明,在将微卫星标记用于群体遗传学或亲缘关系分析之前,需要测试其在对虾中的遗传模式。