p21（WAF1）在骨肉瘤中的表达的预后意义

[Prognostic significance of p21(WAF1) expression in osteosarcoma].

作者信息

Zhang Chun-Lin, Liao Wei-Ming, Li Fo-Bao, Zeng Bing-Fang, Zeng Yi-Xin

机构信息

Department of Orthopedic Surgery, The Sixth Affiliated Hospital of Shanghai Jiaotong University, Shanghai 200233, China.

出版信息

Zhonghua Bing Li Xue Za Zhi. 2005 Aug;34(8):524-7.

PMID:16383301

Abstract

OBJECTIVE

To investigate the expression of p21(WAF1) gene in human osteosarcoma and their relationships with clinicopathological parameters and prognostic value.

METHODS

The p21(WAF1) gene mRNA and p21 protein expression in 45 osteosarcoma and 10 fibrous dysplasia of bone specimens were analyzed by in situ hybridization and immunohistochemistry respectively.

RESULTS

(1) The positive rate of p21 protein expression in osteosarcoma was 17.7% (8/45). (2) The expression rate in high proliferation (4/10) significantly higher than that in low proliferation (4/35) osteosarcoma (chi2 = 4.34, P < 0.05). (3) The positive rate of p21(WAF1) mRNA expression in osteosarcoma was 42.2% (19/45). The expression rate in high proliferation (6/10) significantly higher than that in low proliferation (13/35) osteosarcoma (chi2 = 20.6, P < 0.01). (4) The survival time after operation of the patients with p21(WAF1) mRNA expression were higher than that of the patients with p21(WAF1) mRNA negative expression (P < 0.05).

CONCLUSIONS

(1) With the increase in degree of malignancy, the expression of p21(WAF1) mRNA and p21 protein in osteosarcoma tend decrease. (2) The expression of p21(WAF1) mRNA has a definite value in judging prognosis in osteosarcoma.

摘要

目的

探讨p21（WAF1）基因在人骨肉瘤中的表达及其与临床病理参数的关系和预后价值。

方法

分别采用原位杂交和免疫组织化学方法分析45例骨肉瘤和10例骨纤维异常增殖症标本中p21（WAF1）基因mRNA和p21蛋白的表达。

结果

（1）骨肉瘤中p21蛋白表达阳性率为17.7%（8/45）。（2）高增殖性骨肉瘤（4/10）中的表达率显著高于低增殖性骨肉瘤（4/35）（χ2 = 4.34，P < 0.05）。（3）骨肉瘤中p21（WAF1）mRNA表达阳性率为42.2%（19/45）。高增殖性骨肉瘤（6/10）中的表达率显著高于低增殖性骨肉瘤（13/35）（χ2 = 20.6，P < 0.01）。（4）p21（WAF1）mRNA表达阳性患者术后生存时间高于p21（WAF1）mRNA阴性表达患者（P < 0.05）。