铜绿假单胞菌的群体感应负调控因子RsaL与lasI启动子结合。
The quorum-sensing negative regulator RsaL of Pseudomonas aeruginosa binds to the lasI promoter.
作者信息
Rampioni Giordano, Bertani Iris, Zennaro Elisabetta, Polticelli Fabio, Venturi Vittorio, Leoni Livia
机构信息
Department of Biology, University Roma Tre, Viale G. Marconi 446, 00146, Rome, Italy.
出版信息
J Bacteriol. 2006 Jan;188(2):815-9. doi: 10.1128/JB.188.2.815-819.2006.
Abstract
A mutation in the rsaL gene of Pseudomonas aeruginosa produces dramatically higher amounts of N-acyl homoserine lactone with respect to the wild type, highlighting the key role of this negative regulator in controlling quorum sensing (QS) in this opportunistic pathogen. The DNA binding site of the RsaL protein on the rsaL-lasI bidirectional promoter partially overlaps the binding site of the LasR protein, consistent with the hypothesis that RsaL and LasR could be in binding competition on this promoter. This is the first direct demonstration that RsaL acts as a QS negative regulator by binding to the lasI promoter.
摘要
铜绿假单胞菌rsaL基因的突变相对于野生型产生了显著更多的N-酰基高丝氨酸内酯，突出了这种负调控因子在控制这种机会致病菌群体感应（QS）中的关键作用。RsaL蛋白在rsaL-lasI双向启动子上的DNA结合位点与LasR蛋白的结合位点部分重叠，这与RsaL和LasR可能在该启动子上存在结合竞争的假设一致。这是首次直接证明RsaL通过与lasI启动子结合而作为QS负调控因子发挥作用。
相似文献
1
The quorum-sensing negative regulator RsaL of Pseudomonas aeruginosa binds to the lasI promoter.
J Bacteriol. 2006 Jan;188(2):815-9. doi: 10.1128/JB.188.2.815-819.2006.
2
RsaL provides quorum sensing homeostasis and functions as a global regulator of gene expression in Pseudomonas aeruginosa.
Mol Microbiol. 2007 Dec;66(6):1557-65. doi: 10.1111/j.1365-2958.2007.06029.x.
3
The multi-output incoherent feedforward loop constituted by the transcriptional regulators LasR and RsaL confers robustness to a subset of quorum sensing genes in Pseudomonas aeruginosa.
Mol Biosyst. 2017 Jun 1;13(6):1080-1089. doi: 10.1039/c7mb00040e. Epub 2017 May 3.
4
Functional characterization of the quorum sensing regulator RsaL in the plant-beneficial strain Pseudomonas putida WCS358.
Appl Environ Microbiol. 2012 Feb;78(3):726-34. doi: 10.1128/AEM.06442-11. Epub 2011 Nov 23.
5
The Pseudomonas putida Lon protease is involved in N-acyl homoserine lactone quorum sensing regulation.
BMC Microbiol. 2007 Jul 26;7:71. doi: 10.1186/1471-2180-7-71.
6
Crystal structure of Pseudomonas aeruginosa RsaL bound to promoter DNA reaffirms its role as a global regulator involved in quorum-sensing.
Nucleic Acids Res. 2017 Jan 25;45(2):699-710. doi: 10.1093/nar/gkw954. Epub 2016 Oct 18.
7
Activity of purified QscR, a Pseudomonas aeruginosa orphan quorum-sensing transcription factor.
Mol Microbiol. 2006 Jan;59(2):602-9. doi: 10.1111/j.1365-2958.2005.04960.x.
8
The Pseudomonas quorum-sensing regulator RsaL belongs to the tetrahelical superclass of H-T-H proteins.
J Bacteriol. 2007 Mar;189(5):1922-30. doi: 10.1128/JB.01552-06. Epub 2006 Dec 15.
9
Mechanisms of RsaL mediated tolerance to ciprofloxacin and carbenicillin in Pseudomonas aeruginosa.
Curr Genet. 2019 Feb;65(1):213-222. doi: 10.1007/s00294-018-0863-3. Epub 2018 Jun 27.
10
[Involvement of quorum-sensing in biosynthesis of polyhydroxyalkanoates in Pseudomonas aeruginosa].
Wei Sheng Wu Xue Bao. 2011 Jun;51(6):769-75.
引用本文的文献
1
Identification of the Pseudomonas aeruginosa AgtR-CspC-RsaL pathway that controls Las quorum sensing in response to metabolic perturbation and Staphylococcus aureus.
PLoS Pathog. 2025 Apr 8;21(4):e1013054. doi: 10.1371/journal.ppat.1013054. eCollection 2025 Apr.
2
RsaL is a self-regulatory switch that controls alternative biosynthesis of two AHL-type quorum sensing signals in PA1201.
mLife. 2024 Mar 18;3(1):74-86. doi: 10.1002/mlf2.12113. eCollection 2024 Mar.
3
The collapse of cooperation during range expansion of Pseudomonas aeruginosa.
Nat Microbiol. 2024 May;9(5):1220-1230. doi: 10.1038/s41564-024-01627-8. Epub 2024 Mar 5.
4
Unveiling therapeutic efficacy of extract and multi-targeting phytocompounds from (Forssk.) Brownsey & Jermy against multidrug-resistant .
RSC Adv. 2024 Feb 16;14(9):6096-6111. doi: 10.1039/d3ra08367e. eCollection 2024 Feb 14.
5
The atypical organization of the family genes in AHL-driven quorum-sensing circuits.
J Bacteriol. 2024 Feb 22;206(2):e0043023. doi: 10.1128/jb.00430-23. Epub 2024 Jan 19.
6
The histidine kinase NahK regulates pyocyanin production through the PQS system.
J Bacteriol. 2024 Jan 25;206(1):e0027623. doi: 10.1128/jb.00276-23. Epub 2024 Jan 3.
7
RsaM: a unique dominant regulator of AHL quorum sensing in bacteria.
Microbiology (Reading). 2023 Nov;169(11). doi: 10.1099/mic.0.001417.
8
Natural defense against multi-drug resistant : L. and antibacterial activity.
RSC Adv. 2023 Oct 2;13(41):28773-28784. doi: 10.1039/d3ra03923d. eCollection 2023 Sep 26.
9
The phage-encoded protein PIT2 impacts quorum sensing by direct interaction with LasR.
iScience. 2023 Aug 26;26(10):107745. doi: 10.1016/j.isci.2023.107745. eCollection 2023 Oct 20.
10
Parameters, architecture and emergent properties of the LasI/LasR quorum-sensing circuit.
J R Soc Interface. 2023 Mar;20(200):20220825. doi: 10.1098/rsif.2022.0825. Epub 2023 Mar 15.
本文引用的文献
1
Dual role of response regulator StyR in styrene catabolism regulation.
Appl Environ Microbiol. 2005 Sep;71(9):5411-9. doi: 10.1128/AEM.71.9.5411-5419.2005.
2
The two-component response regulator PprB modulates quorum-sensing signal production and global gene expression in Pseudomonas aeruginosa.
Mol Microbiol. 2005 Jun;56(5):1287-301. doi: 10.1111/j.1365-2958.2005.04612.x.
3
Quorum sensing: the power of cooperation in the world of Pseudomonas.
Environ Microbiol. 2005 Apr;7(4):459-71. doi: 10.1111/j.1462-2920.2005.00769.x.
4
Transcriptome analysis of quorum-sensing regulation and virulence factor expression in Pseudomonas aeruginosa.
Vaccine. 2004 Dec 6;22 Suppl 1:S15-20. doi: 10.1016/j.vaccine.2004.08.011.
5
Promoter specificity in Pseudomonas aeruginosa quorum sensing revealed by DNA binding of purified LasR.
Proc Natl Acad Sci U S A. 2004 Nov 9;101(45):15833-9. doi: 10.1073/pnas.0407229101. Epub 2004 Oct 25.
6
Regulation of the N-acyl homoserine lactone-dependent quorum-sensing system in rhizosphere Pseudomonas putida WCS358 and cross-talk with the stationary-phase RpoS sigma factor and the global regulator GacA.
Appl Environ Microbiol. 2004 Sep;70(9):5493-502. doi: 10.1128/AEM.70.9.5493-5502.2004.
7
Global regulation of quorum sensing and virulence by VqsR in Pseudomonas aeruginosa.
Microbiology (Reading). 2004 Apr;150(Pt 4):831-841. doi: 10.1099/mic.0.26906-0.
8
The role of regulators in the expression of quorum-sensing signals in Pseudomonas aeruginosa.
J Mol Microbiol Biotechnol. 2003;6(2):88-100. doi: 10.1159/000076739.
9
Posttranscriptional control of quorum-sensing-dependent virulence genes by DksA in Pseudomonas aeruginosa.
J Bacteriol. 2003 Jun;185(12):3558-66. doi: 10.1128/JB.185.12.3558-3566.2003.
10
Styrene-catabolism regulation in Pseudomonas fluorescens ST: phosphorylation of StyR induces dimerization and cooperative DNA-binding.
Biochem Biophys Res Commun. 2003 Apr 11;303(3):926-31. doi: 10.1016/s0006-291x(03)00450-9.
Zotero 插件