Sánchez-Camacho Cristina, López Jesús M, González Agustín
Departamento de Biología Celular, Facultad de Biología, Universidad Complutense, 28040 Madrid, Spain.
J Comp Neurol. 2006 Feb 20;494(6):961-75. doi: 10.1002/cne.20833.
The organization of the basal forebrain cholinergic system (BFCS) in the frog was studied by means of choline acetyltransferase (ChAT) immunohistochemistry. The BFCS was observed as a conspicuous cholinergic cell population extending through the diagonal band, medial septal nucleus, bed nucleus of the stria terminalis, and pallidal regions. Abundant fiber labeling was also found around the labeled cell bodies. The combination of retrograde tract tracing with dextran amines and ChAT immunohistochemistry revealed intraseptal and intra-BFCS cholinergic connections. In addition, an extratelencephalic cholinergic input from the laterodorsal tegemental nucleus was demonstrated. The possible influence of monoaminergic inputs on the BFCS neurons was examined by means of tyrosine hydroxylase and serotonin immunohistochemistry combined with ChAT immunolabeling. Our results showed that catecholaminergic fibers overlapped the BFCS, with the exception of the medial septal nucleus. Serotoninergic innervation was widespread, but less abundant in the caudal extent of the BFCS. Taken together, our results on the localization of the cholinergic neurons in the basal forebrain and their relationship with cholinergic, catecholaminergic, and serotoninergic afferents have shown numerous common features with amniotes. In particular, anurans and mammals (for which most data is available) share a strikingly comparable organization pattern of the BFCS.
利用胆碱乙酰转移酶（ChAT）免疫组织化学方法研究了青蛙基底前脑胆碱能系统（BFCS）的组织结构。观察到BFCS是一个明显的胆碱能细胞群，延伸穿过斜角带、内侧隔核、终纹床核和苍白球区域。在标记的细胞体周围也发现了丰富的纤维标记。逆行束路追踪与葡聚糖胺相结合以及ChAT免疫组织化学揭示了隔区内和BFCS内的胆碱能连接。此外，还证明了来自外侧被盖核的脑外胆碱能输入。通过酪氨酸羟化酶和5-羟色胺免疫组织化学结合ChAT免疫标记，研究了单胺能输入对BFCS神经元的可能影响。我们的结果表明，除内侧隔核外，儿茶酚胺能纤维与BFCS重叠。5-羟色胺能神经支配广泛，但在BFCS的尾部区域较少。综上所述，我们关于基底前脑胆碱能神经元的定位及其与胆碱能、儿茶酚胺能和5-羟色胺能传入神经关系的研究结果显示，与羊膜动物有许多共同特征。特别是，无尾两栖类动物和哺乳动物（有最多数据）的BFCS具有惊人的相似组织模式。
