Greer Amy L, Berrill Michael, Wilson Paul J
Watershed Ecosystems Graduate Program, Trent University, 1600 West Bank Dr., Peterborough, Ontario K9J 7B8, Canada.
Dis Aquat Organ. 2005 Nov 9;67(1-2):9-14. doi: 10.3354/dao067009.
Using field, molecular and histological methods, an epizootic, systemic disease causing death within wood frog Rana sylvatica tadpoles and leopard frog Rana pipiens metamorphs at 3 different locations within Southern Ontario, Canada, has been investigated. Our results demonstrated that the probable cause of this disease was a ranavirus. Affected amphibians were found to exhibit necrosis within the hematopoietic cells. Liver tissue samples were found positive for the virus by PCR amplification of the ranavirus (Family: Iridoviridae) major capsid protein (MCP). Positive samples were confirmed by sequence analysis. Clinically normal, laboratory-raised wood frog egg broods were also found to test weakly positive for ranavirus. The population effects of disease on these amphibian communities have not yet been conclusively associated with population declines, but warrant more focused consideration.
利用野外、分子和组织学方法，对加拿大安大略省南部3个不同地点的林蛙蝌蚪和豹蛙幼体中引发一种导致死亡的流行性、全身性疾病进行了调查。我们的结果表明，这种疾病的可能病因是一种蛙病毒。受影响的两栖动物被发现造血细胞出现坏死。通过对蛙病毒（科：虹彩病毒科）主要衣壳蛋白（MCP）进行PCR扩增，发现肝脏组织样本对该病毒呈阳性。阳性样本通过序列分析得到确认。临床正常、在实验室饲养的林蛙卵群也被检测出对蛙病毒呈弱阳性。该疾病对这些两栖动物群落的种群影响尚未最终确定与种群数量下降有关，但值得更深入地研究。
