Arakawa M, Kambara K, Miyazaki H, Segawa T, Ando F, Kawada T, Ohno M, Nakahara K
Second Department of Internal Medicine, Gifu University School of Medicine, Japan.
Clin Ther. 1992 May-Jun;14(3):396-408.
To determine if alloxan-induced lung injury could be prevented by an antiprotease, ulinastatin, we used three groups of five anesthetized, ventilated dogs. They were given saline (20 ml/kg/hr) infusion alone (saline group), alloxan (75 mg/kg) + saline infusion (alloxan group), or ulinastatin (50,000 U/kg) + alloxan + saline infusion (ulinastatin group). The course of all dogs was followed for three hours. In the saline group, extravascular lung water to blood-free dry weight (Qwl/dQl) was 3.22 +/- 0.31 g/g (mean +/- SE). The alloxan group presented the following significant findings: a decrease in white blood cell and platelet counts (44.2% and 68.2% of control, respectively) at five minutes; an increase in thromboxane B2 and 6-keto-prostaglandin F1 alpha (731.6% and 476.6% of control, respectively) at 15 minutes; an increase in beta-glucuronidase (124.8% of control) at 30 minutes; and an increase in Qwl/dQl (8.84 +/- 1.82 g/g) at the end of experiment. The addition of ulinastatin significantly reduced most alloxan-induced effects: differences in white blood cell counts, thromboxane B2, 6-keto-prostaglandin F1 alpha, and Qwl/dQl between the saline and ulinastatin groups were small. We conclude that ulinastatin significantly reduces the extent of lung water accumulation in alloxan-induced lung injury.
为了确定抗蛋白酶乌司他丁是否能够预防四氧嘧啶诱导的肺损伤，我们使用了三组，每组五只麻醉并进行机械通气的犬。它们分别接受单独输注生理盐水（20 ml/kg/hr）（生理盐水组）、四氧嘧啶（75 mg/kg）+生理盐水输注（四氧嘧啶组）或乌司他丁（50,000 U/kg）+四氧嘧啶+生理盐水输注（乌司他丁组）。所有犬的实验过程持续三小时。在生理盐水组中，血管外肺水与去血干重之比（Qwl/dQl）为3.22±0.31 g/g（均值±标准误）。四氧嘧啶组出现了以下显著结果：5分钟时白细胞和血小板计数下降（分别为对照组的44.2%和68.2%）；15分钟时血栓素B2和6-酮-前列腺素F1α增加（分别为对照组的731.6%和476.6%）；30分钟时β-葡萄糖醛酸酶增加（为对照组的124.8%）；实验结束时Qwl/dQl增加（8.84±1.82 g/g）。添加乌司他丁显著减轻了大多数四氧嘧啶诱导的效应：生理盐水组和乌司他丁组在白细胞计数、血栓素B2、6-酮-前列腺素F1α和Qwl/dQl方面的差异很小。我们得出结论，乌司他丁可显著减轻四氧嘧啶诱导的肺损伤中肺水积聚的程度。
