葡萄糖脑苷脂是植物质膜中主要的鞘脂类吗？

Are glucocerebrosides the predominant sphingolipids in plant plasma membranes?

作者信息

Sperling Petra, Franke Stephan, Lüthje Sabine, Heinz Ernst

机构信息

Universität Hamburg, Biozentrum Klein Flottbek und Botanischer Garten, Ohnhorststrasse 18, 22609 Hamburg, Germany.

出版信息

Plant Physiol Biochem. 2005 Dec;43(12):1031-8. doi: 10.1016/j.plaphy.2005.10.004. Epub 2005 Dec 13.

DOI:10.1016/j.plaphy.2005.10.004

PMID:16386430

Abstract

Long-chain sphingobases have been analyzed in various fractions prepared from different organs (leaf, root, storage tissue) from five dicotyledoneous plants (Arabidopsis thaliana, Brassica oleracea, Nicotiana tabacum, Pisum sativum, Spinacia oleracea). The resulting sphingobase profiles from cerebrosides and plasma membranes (PMs) show large qualitative and quantitative differences. Assuming that cerebrosides from all cellular membranes have similar sphingobase profiles, these data suggest that cerebrosides, considered to be characteristic glycolipids of plant PMs and specified by large proportions of sphingobases with an 8Z-double bond motif, do not represent the major sphingolipids of PMs. The fraction of unidentified complex sphingolipids, containing mainly 8E-phytosphingenine, exceeds the cerebroside proportion in PMs by several factors and may be as abundant as diacylglycerol-based phospholipids. These results are discussed with respect to the distribution of various lipids between the bilayer halves of plant PM.

摘要

已对五种双子叶植物（拟南芥、甘蓝、烟草、豌豆、菠菜）不同器官（叶、根、贮藏组织）制备的各种组分中的长链鞘氨醇碱进行了分析。从脑苷脂和质膜（PMs）得到的鞘氨醇碱谱显示出很大的质和量的差异。假设所有细胞膜的脑苷脂具有相似的鞘氨醇碱谱，这些数据表明，被认为是植物PMs特征糖脂且由大量具有8Z-双键基序的鞘氨醇碱所特化的脑苷脂，并不代表PMs的主要鞘脂。主要含有8E-植物鞘氨醇的未鉴定复合鞘脂组分在PMs中的比例比脑苷脂高出几个数量级，其丰富程度可能与基于二酰甘油的磷脂相当。针对植物PM双层膜两半之间各种脂质的分布对这些结果进行了讨论。