Davis J C, Hymer W C
J Endocrinol. 1975 Feb;64(2):229-36. doi: 10.1677/joe.0.0640229.
An assay system, using the characteristic migration of follicle-stimulating hormone (FSH) on polyacrylamide gels, was developed to evaluate the effects of hypothalamic factors on amino acid incorporation into FSH in the rat adenohypophysis incubated in vitro. Follicle-stimulating hormone extracted from rat pituitary secretory granules was found to migrate on 7-5% polyacrylamide gels, pH 9-5, as a protein with an Rf of 0-614. The characteristic Rf was used to measure [14C]amino acid incorpotation into FSH in rat adenohypophyses in vitro. Follicle-stimulating hormone synthesis increased after addition of crude hypothalamic extract to the incubation medium. Hormone synthesis was also increased three times after the addition of dibutyryl cyclic AMP.
利用促卵泡激素（FSH）在聚丙烯酰胺凝胶上的特征性迁移，开发了一种测定系统，以评估下丘脑因子对体外培养的大鼠腺垂体中氨基酸掺入FSH的影响。从大鼠垂体分泌颗粒中提取的促卵泡激素在pH 9.5的7.5%聚丙烯酰胺凝胶上迁移，作为一种Rf为0.614的蛋白质。该特征性Rf用于测量体外培养的大鼠腺垂体中[14C]氨基酸掺入FSH的情况。向孵育培养基中添加粗制下丘脑提取物后，促卵泡激素的合成增加。添加二丁酰环磷腺苷后，激素合成也增加了三倍。
