Hu Jing-bo, Zhou Yan, Jiang Dan-dan, Tan Wen-song
The State Key Laboratory of Bioreactor Engineering, East China University of Sciences and Technology, Shanghai 200237, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Jan;22(1):7-10.
To investigate the effects of ex-vivo expansion on proliferative ability, pluripotentiality and other biologic characteristics of human bone marrow mesenchymal stem cells(MSCs).
MSCs were isolated from human costal bone and passaged under the same culture conditions. At each passage, the characteristics of proliferation kinetics, osteogenic, chondrogenic, adipogenic differentiation potential were analyzed, and cell morphology, surface markers and cell cycle were investigated as well.
The proliferative ability and osteogenic, adipogenic differentiation potential decreased during culture expansion, while chondrogenic differentiation potential had no significant changes. MSCs maintained their multiple differentiation potential during their life-span. For each passage, the positive ratio of CD29, CD44, CD105 were all above 90% and the negative ratio of CD14, CD34, CD45 were below 4%.
Culture expansion causes MSCs to gradually lose their stem cell properties. During ex-vivo expansion of hMSCs, the osteogenic and adipogenic differentiation potential are more likely to lose than chondrogenic differentiation potential. Multiple differentiation potential is conserved longer than self-renewal capacity. MSCs before 7th passage can be a valuable subject for basic research and clinical application.
探讨体外扩增对人骨髓间充质干细胞（MSCs）增殖能力、多能性及其他生物学特性的影响。
从人肋骨中分离MSCs，并在相同培养条件下传代。每次传代时，分析增殖动力学、成骨、成软骨、成脂分化潜能的特征，并研究细胞形态、表面标志物和细胞周期。
培养扩增过程中，增殖能力及成骨、成脂分化潜能下降，而成软骨分化潜能无明显变化。MSCs在其寿命期间保持其多向分化潜能。每次传代时，CD29、CD44、CD105的阳性率均高于90%，CD14、CD34、CD45的阴性率均低于4%。
培养扩增导致MSCs逐渐丧失其干细胞特性。在人MSCs的体外扩增过程中，成骨和成脂分化潜能比成软骨分化潜能更容易丧失。多向分化潜能比自我更新能力保留的时间更长。第7代之前的MSCs可为基础研究和临床应用提供有价值的研究对象。
