[RDP1258 on proliferation of rat splenocytes in vivo].

AIM

To explore the immunosuppressive effects of a novel HLA-derived peptide, RDP1258, on rat splenocyte proliferation in vivo and its underlying mechanisms.

METHODS

RDP1258 was synthesized through artificial solid-phase synthesis method. Its effects on proliferative reaction of rat splenocytes in vivo were observed by (3)H-TDR incorporation method. And its effects on the heme oxygenase(HO) enzyme activity were analyzed by enzyme chemistry method.

RESULTS

The results showed that the synthetic HLA-derived peptide RDP1258 obviously inhibited the proliferation of rat splenocytes stimulated by isoantigen and mitogen. In vivo administration of RDP1258 peptide to rats resulted in upregulation of splenic HO activity and rapid upregulation of HO-1 protein expression.

CONCLUSION

HO-1 might participate in RDP1258 inhibiting the proliferation of rat splenocytes induced by mitogen and isoantigen in vivo.