抗HER-2嵌合抗体chA21对SKOV3细胞增殖抑制及诱导凋亡的作用
[Effects of anti-HER-2 chimeric antibody chA21 on proliferative inhibition of SKOV3 cells and inducing their apoptosis].
作者信息
Xue Hua, Wu Qiang, Hu Xiang-yang, Ling Xiao-guang, Cheng Lian-sheng, Liu Jing, Liu Ai-ping
机构信息
Department of Pathology, Anhui Medical University, Hefei 230032, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Jan;22(1):51-3, 57.
AIM
To explore the effects of anti-HER-2 chimeric antibody chA21 on proliferation and apoptosis of ovarian cancer cell lines SKOV3.
METHODS
MTT colorometric assay, HE staining, transmission electron microscopy, flow cytometry and TUNEL staining were used to study the proliferative inhibition and apoptotic induction of SKOV3 cells by chA21 in vitro.
RESULTS
Proliferative inhibition rate and apoptotic rate of SKOV3 cells were increased with dose and action time by chA21 (0.2 mg/L-5.4 mg/L).
CONCLUSION
chA21 can remarkably inhibit proliferation of SKOV3 cells in vitro and induction of apoptosis may be a principal way.
目的
探讨抗HER-2嵌合抗体chA21对卵巢癌细胞系SKOV3增殖和凋亡的影响。
方法
采用MTT比色法、HE染色、透射电子显微镜、流式细胞术和TUNEL染色研究chA21体外对SKOV3细胞的增殖抑制和凋亡诱导作用。
结果
chA21（0.2mg/L - 5.4mg/L）可使SKOV3细胞的增殖抑制率和凋亡率随剂量和作用时间增加。
结论
chA21可显著抑制SKOV3细胞的体外增殖，诱导凋亡可能是其主要作用方式。
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