[运用四聚体技术定量分析原发性胆汁性肝硬化患者外周血中抗原特异性T淋巴细胞]

[Quantitation of antigen specific T lymphocytes in peripheral blood of patients with primary biliary cirrhosis using tetramer technology].

作者信息

Liu Hai-ying, Zhang Jian, Deng An-mei, Geng Hong-lian, Zhou Lin, Zhu Ye, Xu De-xing, Zhong Ren-qian

机构信息

Clinical Laboratory, General Hospital of Guangzhou, Guangzhou 510010, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Jan;22(1):78-81.

PMID:16388752

Abstract

AIM

To quantitate antigen specific T lymphocytes in peripheral blood from patients with primary biliary cirrhosis(PBC) and study the role of antigen specific T lymphocytes in the development of PBC.

METHODS

Using tetramers and CD8 monoclonal antibody staining, PDC-E2 159-167aa and PDC-E2 165-174aa specific CD8(+) T lymphocytes were determined respectively in the peptide-induced cytotoxic T cell lines prepared from peripheral blood mononuclear cells(PBMC) of 15 PBC patients. The frequencies of these two kinds of antigen specific T lymphocytes in HLA-A*0201 positive (A2(+)) PBC were compared with those in A2(-) PBC patients, patients with other A2(+) chronic liver diseases and healthy controls.

RESULTS

PDC-E2 159-167aa/HLA-A0201 and PDC-E2 165-174aa/HLA-A0201 tetramer positive CD8(+) T lymphocytes were detected in all of A2(+) PBC patients with average percentages of 0.42%+/-0.24% (0.17%-1.08%) and 0.27%+/-0.17% (0.05%-0.56%), respectively. The frequencies of the two kinds of antigen specific CD8(+) T lymphocytes from peripheral blood were significantly higher in earlier stages I and II of PBC as compared with stage III (P<0.001), while no difference was found between PDC-E2 159-167aa and PDC-E2 165-174aa specific CD8(+) T lymphocytes at the same stages. In addition, there existed no statistical difference between frequencies of antigen specific T lymphocytes in AMA or anti-PDC positive and negative PBC patients (P>0.05).

CONCLUSION

This study suggests that HLA-A*0201 restricted PDC-E2 165-174aa and PDC-E2 159-167aa specific CTL play important roles in the development of PBC, and there might be a similar mechanism of T cell-mediated damage between AMA or anti-PDC positive and negative PBC patients.

摘要

目的

定量分析原发性胆汁性肝硬化（PBC）患者外周血中抗原特异性T淋巴细胞，并研究抗原特异性T淋巴细胞在PBC发病中的作用。

方法

采用四聚体和CD8单克隆抗体染色，分别检测15例PBC患者外周血单个核细胞（PBMC）制备的肽诱导细胞毒性T细胞系中PDC-E2 159-167aa和PDC-E2 165-174aa特异性CD8(+) T淋巴细胞。比较HLA-A*0201阳性（A2(+)）PBC患者与A2(-) PBC患者、其他A2(+)慢性肝病患者及健康对照者外周血中这两种抗原特异性T淋巴细胞的频率。

结果

在所有A2(+) PBC患者中均检测到PDC-E2 159-167aa/HLA-A0201和PDC-E2 165-174aa/HLA-A0201四聚体阳性CD8(+) T淋巴细胞，平均百分比分别为0.42%±0.24%（0.17%-1.08%）和0.27%±0.17%（0.05%-0.56%）。与III期相比，PBC I期和II期外周血中这两种抗原特异性CD8(+) T淋巴细胞的频率显著更高（P<0.001），而同一阶段PDC-E2 159-167aa和PDC-E2 165-174aa特异性CD8(+) T淋巴细胞之间无差异。此外，AMA或抗PDC阳性和阴性PBC患者的抗原特异性T淋巴细胞频率之间无统计学差异（P>0.05）。