Ishikawa T, Kakumu S, Wakita T, Takayanagi M, Yoshioka K
Third Department of Internal Medicine, Nagoya University School of Medicine, Japan.
J Clin Lab Immunol. 1991 May;35(1):17-25.
Peripheral blood mononuclear cells from patients with primary biliary cirrhosis (PBC) were studied for their ability to produce antibodies to mitochondrial antigens (antimitochondrial antibody, AMA and anti-pyruvate dehydrogenase complex, anti-PDC), using dot-immunobinding and ELISA assay. 2 x 10(6) B cells alone and the mixture of 1.4 x 10(6) T plus 0.6 x 10(6) B cells from patients with serum AMA+ PBC spontaneously produced similar amounts of AMA and anti-PDC. Coculture experiments of T and B cells showed that anti-PDC production was significantly greater in pokeweed mitogen (PWM)-stimulated cultures than in unstimulated ones. The reactivities of antibodies to mitochondrial antigens were inhibited by M2 mitochondrial antigen-enriched fraction. Ursodeoxycholic acid added to cultures resulted in a significant inhibition for production of immunoglobulins and anti-PDC (IgG and IgM). Spontaneous production of AMA or anti-PDC was not detected in patients with AMA- PBC, chronic viral hepatitis and healthy controls, nor could it be induced by PWM in either group. EBV-transformed B cell line established from a AMA+ PBC patient produced high levels of AMA and IgM class anti-PDC. These findings indicate that antibody to a specific mitochondrial antigen in patients with PBC may be produced in T cell-dependent and-independent manner.
利用斑点免疫结合法和酶联免疫吸附测定法，对原发性胆汁性肝硬化（PBC）患者的外周血单个核细胞产生抗线粒体抗原抗体（抗线粒体抗体，AMA以及抗丙酮酸脱氢酶复合体，抗-PDC）的能力进行了研究。来自血清AMA阳性PBC患者的2×10⁶个单独的B细胞以及1.4×10⁶个T细胞与0.6×10⁶个B细胞的混合物自发产生了相似数量的AMA和抗-PDC。T细胞与B细胞的共培养实验表明，在商陆有丝分裂原（PWM）刺激的培养物中抗-PDC的产生明显多于未刺激的培养物。富含M2线粒体抗原的组分可抑制抗体与线粒体抗原的反应性。向培养物中添加熊去氧胆酸可显著抑制免疫球蛋白和抗-PDC（IgG和IgM）的产生。在AMA阴性PBC患者、慢性病毒性肝炎患者及健康对照者中未检测到AMA或抗-PDC的自发产生，两组中PWM也无法诱导其产生。从一名AMA阳性PBC患者建立的EB病毒转化B细胞系产生了高水平的AMA和IgM类抗-PDC。这些发现表明，PBC患者中针对特定线粒体抗原的抗体可能以T细胞依赖和非依赖的方式产生。
