Kang Jung-Hoon, Kim Soo-A, Hong Kyong-Ja
Department of Biochemistry, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, Seoul 137-701, Republic of Korea.
FEBS Lett. 2006 Jan 23;580(2):510-6. doi: 10.1016/j.febslet.2005.12.065. Epub 2005 Dec 28.
Thrombospondin-1 (TSP1) expression has previously been shown to be regulated primarily at the level of transcription. In the present study, transcriptional control was not involved in the induction of TSP1 by heat shock. In contrast, heat shock caused significant stabilization of TSP1 mRNA. Fusion of the 3'-untranslated region (UTR) of TSP1 mRNA, with a reporter gene, increased the stability of the reporter transcript by heat shock. Furthermore, we identified a putative region from 968 to 1258 from the stop codon in the TSP1 3'-UTR, involved in the stability by heat shock. Thus, the induction of TSP1 by heat shock may occur through a post-transcriptional mechanism.
血小板反应蛋白-1（TSP1）的表达先前已被证明主要在转录水平受到调控。在本研究中，热休克诱导TSP1的过程不涉及转录控制。相反，热休克导致TSP1 mRNA显著稳定。TSP1 mRNA的3'非翻译区（UTR）与报告基因融合，通过热休克增加了报告转录本的稳定性。此外，我们在TSP1 3'-UTR中从终止密码子起鉴定出一个968至1258的假定区域，其参与热休克介导的稳定性调节。因此，热休克诱导TSP1可能是通过转录后机制发生的。
