Kirakosyan Ara, Kaufman Peter, Nelson Randall L, Kasperbauer Michael J, Duke James A, Seymour Elisabeth, Chang Soo Chul, Warber Sara, Bolling Steven
University of Michigan Integrative Medicine Program, University of Michigan, Ann Arbor, Michigan 48104, USA.
J Agric Food Chem. 2006 Jan 11;54(1):54-8. doi: 10.1021/jf052458w.
The objective of the present study was to determine whether concentrations of different isoflavones (puerarin, genistein, genistin, daidzein, and daidzin) in shoots and roots of five selected soybean genotypes would respond the same or differently to red (650 nm peak transmittance) and far-red (750 nm peak transmittance) light treatments given under controlled environments. Levels of isoflavones (mg g(-1) dry weight biomass) present in seeds, control roots, and shoots and 10 day light-treated seedlings (light, dark, red, and far-red wavelengths) of soybean (Glycine max) were determined by high-performance liquid chromatography analysis in comparison with known isoflavone standards. Seeds of the five soybean genotypes studied consistently stored most of their isoflavones as glucosyl conjugates (e.g., daidzin, genistin, and puerarin). For the five soybean genotypes, isoflavone levels were lower in the seeds as compared with roots plus shoots of control, time zero (first true leaf stage) seedlings. Following 10 days of the respective light treatments, we found that (i) isoflavone levels were enhanced in dark-grown plants over light-grown plants for three of the five genotypes (a new finding) and the reverse occurred for a single genotype (a typical response of legumes) and (ii) generally, far-red end of day (EOD) light treatment enhanced total isoflavone levels in roots plus shoots over red EOD light treatment. Results from the present study show that phytochrome does appear to play a role in regulating isoflavone levels in developing soybean seedlings and that this influence by red/far-red-mediated phytochrome reactions is strongly dependent on the genotypes selected for study.
本研究的目的是确定在可控环境下，五种选定大豆基因型的地上部和根部中不同异黄酮（葛根素、染料木黄酮、染料木苷、大豆苷元、大豆苷）的浓度对红色（峰值透光率650nm）和远红色（峰值透光率750nm）光处理的反应是否相同或不同。通过高效液相色谱分析，与已知异黄酮标准品相比，测定了大豆（Glycine max）种子、对照根、地上部以及10天光照处理幼苗（光照、黑暗、红色和远红色波长）中异黄酮的含量（mg g(-1)干重生物量）。所研究的五种大豆基因型的种子始终将其大部分异黄酮以葡萄糖苷共轭物（如大豆苷、染料木苷和葛根素）的形式储存。对于这五种大豆基因型，与对照（零时间，第一片真叶期）幼苗的根和地上部相比，种子中的异黄酮含量较低。在进行了10天的相应光照处理后，我们发现：（i）对于五种基因型中的三种，黑暗生长的植物中异黄酮含量高于光照生长的植物（一项新发现），而对于单一基因型则相反（豆科植物的典型反应）；（ii）一般来说，与红色日终（EOD）光照处理相比，远红色EOD光照处理提高了根和地上部的总异黄酮含量。本研究结果表明，光敏色素似乎确实在调节发育中的大豆幼苗异黄酮含量方面发挥作用，并且这种由红/远红介导的光敏色素反应的影响强烈依赖于所选择用于研究的基因型。
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