Phommalth Siviengkhek, Jeong Yeon-Shin, Kim Yong-Hoon, Dhakal Krishna Hari, Hwang Young-Hyun
School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea.
J Agric Food Chem. 2008 Nov 12;56(21):10123-8. doi: 10.1021/jf802118g. Epub 2008 Oct 9.
Our research objective was to increase isoflavone content in the germinated soybean seeds of four different varieties (Pungsannamulkong, Cheongjakong, Aga4, and Aga3) by optimizing light treatments (dark, greenhouse, fluorescent, incandescent, and ultraviolet lamps). The results demonstrated that the highest isoflavone content was produced from the Aga3 variety, which was developed by an interspecific cross between Eunhakong (Glycine max) and KLG10084 (G. soja) at the Plant Genetic Laboratory, Kyunpook National University. Aga3 is known to have one of the highest isoflavone content in the world at present. Our results recommend exposure of 7-day-old Aga3 sprouts to a combined light treatment of greenhouse lamps (12 h per day) and ultraviolet light (40 min per day) for maximum isoflavone production. Aga3 produced high levels of isoflavone because, in part, it contained very high isoflavone levels within the seed as compared with the other varieties. Under stress conditions, Aga3 could produce over 1.90 times more isoflavone than its seed content and 1.53 times more isoflavone than when grown under dark conditions.
我们的研究目标是通过优化光照处理（黑暗、温室、荧光灯、白炽灯和紫外线灯）来提高四个不同品种（丰山黑豆、清家空、阿加4和阿加3）发芽大豆种子中的异黄酮含量。结果表明，异黄酮含量最高的是阿加3品种，它是由庆北国立大学植物遗传实验室通过银合欢（大豆）和KLG10084（野生大豆）种间杂交培育而成。目前已知阿加3是世界上异黄酮含量最高的品种之一。我们的研究结果建议，将7日龄的阿加3豆芽置于温室灯（每天12小时）和紫外线（每天40分钟）的组合光照处理下，以实现异黄酮的最大产量。阿加3能产生高水平的异黄酮，部分原因是与其他品种相比，其种子中含有非常高的异黄酮水平。在胁迫条件下，阿加3产生的异黄酮比其种子含量高出1.90倍以上，比在黑暗条件下生长时高出1.53倍。
