Yamashita Akihiro, Takada Tatsuyuki, Narita Junko, Yamamoto Gaku, Torii Ryuzo
Department of Oral and Maxillofacial Surgery, Research Center for Animal Life Science, Shiga University of Medical Science, Seta, Ohtsu, Japan.
Cloning Stem Cells. 2005;7(4):232-7. doi: 10.1089/clo.2005.7.232.
Monkey embryonic stem (ES) cell is a useful tool for preclinical studies of regenerative medicine. In this paper, we investigated whether monkey ES cells can be differentiated into osteoblasts in vitro using factors known to promote osteogenesis. We prepared embryoid bodies (EB) in the presence of retinoic acid (RA) and subsequently differentiated in the medium containing either dexamethasone (DEX) or bone morphogenetic protein (BMP)-2 in addition to osteogenic supplements (OS), specifically ascorbic acid and beta-glycerophosphate. RA treatment during EB formation induced osteoblastic marker genes, such as collagen type 1, osteopontin, and Cbfa1. For the expression of osteocalcin, however, cultivation with medium containing either DEX or BMP-2 in addition to OS was required. These results showed that osteoblasts could be derived from monkey ES cells in vitro and BMP-2 + OS was effective to induce calcification.
猴胚胎干细胞是再生医学临床前研究的一种有用工具。在本文中，我们研究了利用已知促进成骨的因子，猴胚胎干细胞在体外是否能分化为成骨细胞。我们在视黄酸（RA）存在的情况下制备胚状体（EB），随后在含有除成骨补充剂（OS）（具体为抗坏血酸和β-甘油磷酸）之外的地塞米松（DEX）或骨形态发生蛋白（BMP）-2的培养基中进行分化。在EB形成过程中进行RA处理可诱导成骨细胞标记基因的表达，如I型胶原、骨桥蛋白和Cbfa1。然而，对于骨钙素的表达，除了OS外，还需要在含有DEX或BMP-2的培养基中培养。这些结果表明，成骨细胞可在体外从猴胚胎干细胞中获得，并且BMP-2 + OS对诱导钙化有效。
