Venuta S, Rubin H
J Natl Cancer Inst. 1975 Feb;54(2):395-400.
We studied the effect of glucose starvation on glucose uptake and thymidine uptake and incorporation in cultures of normal chicken embryo cells and those transformed by Rous sarcoma virus. Resting normal fibroblasts increased the rate of glucose transport up to tenfold when they were starved for glucose, whereas fast-growing normal cells doubled the rate of uptake after starvation. Transformed cells did not show any change in the rate of glucose uptake during starvation. Thymidine uptake and incorporation by normal and transformed cells were not affected by glucose starvation. These results showed that a decrease in the glucose concentration of the medium induced a specific increase in the rate of glucose transport by normal chick fibroblasts, but did not change the transport of glucose by transformed cells. Therefore, it is suggested that glucose or one of its metabolic products regulated the hexose uptake of normal chick fibroblasts. Virus-transformed cells were insensitive to this regulation.
我们研究了葡萄糖饥饿对正常鸡胚细胞培养物以及经劳氏肉瘤病毒转化的细胞培养物中葡萄糖摄取、胸苷摄取及掺入的影响。静止的正常成纤维细胞在葡萄糖饥饿时，葡萄糖转运速率提高至原来的十倍，而快速生长的正常细胞在饥饿后摄取速率翻倍。转化细胞在饥饿期间葡萄糖摄取速率未显示任何变化。正常细胞和转化细胞的胸苷摄取及掺入不受葡萄糖饥饿影响。这些结果表明，培养基中葡萄糖浓度的降低会导致正常鸡成纤维细胞的葡萄糖转运速率特异性增加，但不会改变转化细胞的葡萄糖转运。因此，提示葡萄糖或其代谢产物之一调节了正常鸡成纤维细胞的己糖摄取。病毒转化细胞对这种调节不敏感。
