Weber M J, Evans P K, Johnson M A, McNair T F, Nakamura K D, Salter D W
Fed Proc. 1984 Jan;43(1):107-12.
Transport rates of a number of nutrients and ions have been surveyed in chicken embryo fibroblasts that were density inhibited, growing exponentially, or transformed by Rous sarcoma virus. All the transport systems examined displayed changes associated with changes in growth rate. The rate of ouabain-sensitive potassium transport declined in density-inhibited cells, and increased rapidly in response to serum stimulation. This transport system was regulated both by changes in the activity of the transporters and by the number of transporters in the cell membrane. The rate of transport of the amino acid analog alpha-aminoisobutyric acid declined when cells became density inhibited, but also showed alterations in regulation that were associated with malignant transformation. The rate of glucose transport displayed both growth state-related and transformation-specific changes. The increased rate of glucose transport seen in transformed cells is due to an increase in the number of glucose transporters in the cell membrane. Increased glucose transport is necessary for subsequent changes in glycolysis, and temporally precedes some of the changes in activity of glycolytic enzymes.
已对鸡胚成纤维细胞中多种营养物质和离子的转运速率进行了研究，这些细胞处于密度抑制状态、指数生长状态或被劳氏肉瘤病毒转化。所有检测的转运系统都表现出与生长速率变化相关的改变。哇巴因敏感的钾转运速率在密度抑制的细胞中下降，而在血清刺激后迅速增加。该转运系统既受转运体活性变化的调节，也受细胞膜上转运体数量的调节。当细胞变得密度抑制时，氨基酸类似物α-氨基异丁酸的转运速率下降，但也表现出与恶性转化相关的调节改变。葡萄糖转运速率显示出与生长状态相关以及转化特异性的变化。在转化细胞中观察到的葡萄糖转运速率增加是由于细胞膜上葡萄糖转运体数量的增加。葡萄糖转运增加对于糖酵解的后续变化是必要的，并且在时间上先于糖酵解酶活性的一些变化。
