Johansson Anders, Koskiniemi Satu, Gottfridsson Per, Wiström Johan, Monsen Tor
Department of Clinical Microbiology, Infectious Diseases, Umeå University, Sweden.
J Clin Microbiol. 2006 Jan;44(1):260-5. doi: 10.1128/JCM.44.1.260-265.2006.
We applied a high-resolution PCR-based typing method, multiple-locus variable-number tandem repeat analysis (MLVA), for discrimination of 30 multidrug-resistant clinical isolates of Staphylococcus epidermidis. The results of MLVA were congruent with results obtained by pulsed-field gel electrophoresis (PFGE). MLVA generated discrete character data, and its discriminatory capacity was comparable to that of PFGE.
我们应用了一种基于高分辨率PCR的分型方法——多位点可变数目串联重复序列分析(MLVA),来鉴别30株耐多药的表皮葡萄球菌临床分离株。MLVA的结果与脉冲场凝胶电泳(PFGE)获得的结果一致。MLVA产生离散的特征数据,其鉴别能力与PFGE相当。