Price Claire E, Reid Sharon J, Driessen Arnold J M, Abratt Valerie R
Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, 7701 Cape Town, South Africa.
Appl Environ Microbiol. 2006 Jan;72(1):923-6. doi: 10.1128/AEM.72.1.923-926.2006.
Preexposure of Bifidobacterium longum NCIMB 702259T to cholate caused increased resistance to cholate, chloramphenicol, and erythromycin. The B. longum ctr gene, encoding a cholate efflux transporter, was transformed into the efflux-negative mutant Escherichia coli KAM3, conferring resistance to bile salts and other antimicrobial compounds and causing the efflux of [14C]cholate.
将长双歧杆菌NCIMB 702259T预先暴露于胆酸盐可导致其对胆酸盐、氯霉素和红霉素的抗性增加。编码胆酸盐外排转运蛋白的长双歧杆菌ctr基因被导入外排缺陷型突变体大肠杆菌KAM3中,赋予其对胆盐和其他抗菌化合物的抗性,并导致[14C]胆酸盐的外排。