Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, UK.
Department of Bioengineering, Stanford University.
Nat Rev Microbiol. 2017 Oct;15(10):606-620. doi: 10.1038/nrmicro.2017.79. Epub 2017 Aug 14.
It is remarkable how robustly a bacterial species can maintain its preferred size. This capacity is intimately related to control of the cell cycle: cell size and growth rate determine the duration of the cell cycle, which must accommodate the initiation and completion of DNA replication, and the assembly of the division apparatus during steady growth. Although we still lack an integrated view of the interconnections among events in the cell cycle, cell growth and cell size, the development of high-throughput imaging and image-processing protocols has stimulated a renaissance in the field. In this Review, we summarize recent findings, present simple classic models for cell size control, introduce high-throughput data-collection techniques, and explore the mechanisms that coordinate cell size with essential growth and cell cycle processes.
令人惊讶的是，一个细菌物种能够多么稳健地维持其偏好的大小。这种能力与细胞周期的控制密切相关：细胞大小和生长速度决定了细胞周期的持续时间，细胞周期必须适应 DNA 复制的起始和完成，以及在稳定生长期间分裂装置的组装。尽管我们仍然缺乏对细胞周期、细胞生长和细胞大小中事件之间相互联系的综合观点，但高通量成像和图像处理协议的发展激发了该领域的复兴。在这篇综述中，我们总结了最近的发现，提出了用于细胞大小控制的简单经典模型，介绍了高通量数据收集技术，并探讨了将细胞大小与必要的生长和细胞周期过程协调的机制。
