Vladimirov Iu A, Farkhutdinov R R, Molodenkov M N
Vopr Med Khim. 1976 Mar-Apr;22(2):216-23.
On incubation of diluted blood serum with ferrous ions kinetics of chemiluminescence, typical for lipid containing systems, was observed. It comprised several sequental steps: quick flash, inhibition of the chemiluminescence, slow flash with subsequent steady-state luminescence. At each step of the phenomenon a correlation was found between the intensity of chemiluminescence, the rate of accumulation of products of lipid peroxidation (malone dialdehyde) and concentration of Fe2+ in the mixture. The data obtained suggest that chemiluminescence of blood serum in presence of Fe2+ is due to lipid peroxidation in the serum. Kinetics of the reaction and shape of the chemiluminiscence curve depended upon a number of factors: dilution of blood serum, concentration of Fe2+ added, temperature, pH and (if blood plasma was used) type of an anticoagulant use. Only in blood lipoproteins, mainly in beta-lipoproteins, the distinctive pattern of peroxidation was developed with simultaneous accumulation of malone dialdehyde, oxidation of ferrous ions and ultralow chemiluminescence. In protein fraction of blood serum addition of Fe2+ caused only the quick splash of chemiluminescence without the malone dialdehyde production.
在将稀释的血清与亚铁离子一起孵育时，观察到了含脂质体系典型的化学发光动力学。它包括几个连续的步骤：快速闪光、化学发光的抑制、随后是稳态发光的缓慢闪光。在该现象的每个步骤中，均发现化学发光强度、脂质过氧化产物（丙二醛）的积累速率与混合物中Fe2+浓度之间存在相关性。所获得的数据表明，存在Fe2+时血清的化学发光是由于血清中的脂质过氧化所致。反应动力学和化学发光曲线的形状取决于许多因素：血清的稀释度、添加的Fe2+浓度、温度、pH值以及（如果使用血浆）所使用的抗凝剂类型。仅在血液脂蛋白中，主要是在β-脂蛋白中，出现了独特的过氧化模式，同时伴有丙二醛的积累、亚铁离子的氧化和超低化学发光。在血清的蛋白质部分中，添加Fe2+仅引起化学发光的快速闪现，而不产生丙二醛。
