在来自正常甲状腺和甲状腺肿的RNA中鉴定一种次要的Tg mRNA转录本。

Identification of a minor Tg mRNA transcript in RNA from normal and goitrous thyroids.

作者信息

Targovnik H M, Cochaux P, Corach D, Vassart G

机构信息

Catedra de Genética y Biologia Molecular, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina.

出版信息

Mol Cell Endocrinol. 1992 Mar;84(1-2):R23-6. doi: 10.1016/0303-7207(92)90087-m.

DOI:10.1016/0303-7207(92)90087-m

PMID:1639210

Abstract

Polymerase chain reaction (PCR) amplification of nt 4502 to nt 5184 of the thyroglobulin (Tg) mRNA from several patients, with or without elevated serum thyrotropin (TSH), showed a predominant fragment of the expected size (683 bp) and a minor fragment of 512 bp. The sequence of this minor fragment revealed that 171 bp were missing between position 4567 and 4737. It is highly probable that the deleted sequence corresponds to a complete exon, suggesting an alternative splicing as mechanism for the generation of the minor transcript.

摘要

对若干患者（血清促甲状腺激素（TSH）升高或未升高）的甲状腺球蛋白（Tg）mRNA的4502位核苷酸至5184位核苷酸进行聚合酶链反应（PCR）扩增，结果显示，主要片段为预期大小（683 bp），还有一个512 bp的小片段。该小片段的序列显示，在4567位和4737位之间缺失了171 bp。缺失序列很可能对应一个完整的外显子，提示可变剪接是产生该小转录本的机制。