(-)-(7R, 8S)-二氢脱氢二松柏醇诱导PC12细胞神经突生长

Induction of neurite outgrowth by (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol from PC12 cells.

作者信息

Shin Jung Soo, Kim Yu Mi, Hong Seong Su, Kang Ho Sang, Yang Yoo Jung, Lee Don Koo, Hwang Bang Yeon, Ro Jai Seup, Lee Myung Koo

机构信息

College of Pharmacy, Chungbuk National University, Cheongju, Korea.

出版信息

Arch Pharm Res. 2005 Dec;28(12):1337-40. doi: 10.1007/BF02977898.

DOI:10.1007/BF02977898

PMID:16392665

Abstract

A lignan derivative, (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol (DHDA), was isolated from Kalopanax septemlobus L. and was observed to have neuritogenic activity. DHDA at 50 microM caused a marked induction of neurite outgrowth and an enhancement of nerve growth factor (NGF)-mediated neurite outgrowth from PC12 cells. However, it did not exhibit any neurotrophic action. At 50 microM, DHDA enhanced NGF-induced neurite-bearing activity. This activity was partially blocked by the mitogen-activated protein kinase (MAPK) inhibitor PD98059 and by GF109203X, a protein kinase C (PKC) inhibitor. These results suggest that DHDA can induce neurite outgrowth and enhance NGF-induced neurite outgrowth from PC12 cells by amplifying up-stream steps such as MAPK and PKC.

摘要

从刺楸中分离出一种木脂素衍生物，(-)-(7R, 8S)-二氢脱氢二松柏醇（DHDA），并观察到其具有促神经突生长活性。50微摩尔的DHDA可显著诱导PC12细胞的神经突生长，并增强神经生长因子（NGF）介导的神经突生长。然而，它未表现出任何神经营养作用。在50微摩尔时，DHDA增强了NGF诱导的含神经突活性。丝裂原活化蛋白激酶（MAPK）抑制剂PD98059和蛋白激酶C（PKC）抑制剂GF109203X可部分阻断该活性。这些结果表明，DHDA可通过放大诸如MAPK和PKC等上游步骤来诱导PC12细胞的神经突生长并增强NGF诱导的神经突生长。