党参总生物碱增强神经生长因子诱导PC12细胞的神经突生长。
Codonopsis pilosula (Franch) Nannf total alkaloids potentiate neurite outgrowth induced by nerve growth factor in PC12 cells.
作者信息
Liu Jian-Hui, Bao Yong-Ming, Song Ji-Jun, An Li-Jia
机构信息
Department of Biochemical Engineering, Dalian University of Technology, Dalian 116024, China.
出版信息
Acta Pharmacol Sin. 2003 Sep;24(9):913-7.
AIM
To explore the effect of Codonopsis pilosula (Franch) Nannf total alkaloids (DSA) on differentiation induced by nerve growth factor (NGF) in PC12 cells.
METHODS
After culturing PC12 cells with DSA in the presence or absence of NGF, neurite outgrowth in PC12 cells and correlated protein kinases were assayed.
RESULTS
DSA alone did not exhibit neuritogenic activity, but caused a significant enhancement of NGF (2 microg/L)-induced neurite outgrowth in PC12 cells, and increased the phosphorylation of mitogen-activated protein kinase (MAPK). Furthermore, this enhancing effect was completely blocked by a specific MAPK kinase inhibitor, PD98059.
CONCLUSION
DSA enhanced the NGF-induced neurite outgrowth in PC12 cells by amplifying an up-stream step of the MAPK-dependent signaling pathway.
目的
探讨党参总生物碱（DSA）对神经生长因子（NGF）诱导PC12细胞分化的影响。
方法
在有或无NGF存在的情况下，用DSA培养PC12细胞，检测PC12细胞的神经突生长及相关蛋白激酶。
结果
单独使用DSA不表现出促神经突生长活性，但能显著增强NGF（2微克/升）诱导的PC12细胞神经突生长，并增加丝裂原活化蛋白激酶（MAPK）的磷酸化。此外，这种增强作用被特异性的MAPK激酶抑制剂PD98059完全阻断。
结论
DSA通过放大MAPK依赖信号通路的上游步骤来增强NGF诱导的PC12细胞神经突生长。
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