高速逆流色谱法从板蓝根中分离纯化出紫花前胡苷B和靛玉红苷A

Peng Jinyong, Fan Guorong, Wu Yutian

Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China.

J Chromatogr A. 2005 Oct 14;1091(1-2):89-93. doi: 10.1016/j.chroma.2005.07.072.

A preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of ethyl acetate-n-butanol-water (2:7:9, v/v/v) was successfully performed to isolate and separate clemastanin B and indigoticoside A from the plant of Radix Isatidis, a traditional Chinese medicine. A total of 59.2 mg clemastanin B and 66.1 mg indigoticoside A with purities of 94.6% and 99.0% determined by high performance liquid chromatography (HPLC) were obtained in one-step elution from 250 mg crude extract, which contained clemastanin B 24.8% and indigoticoside A 28.4%, and the recoveries of clemastanin B and indigoticoside A were 90.3% and 92.2%, respectively. The chemical structure was identified by IR, MS, 1H NMR and 13C NMR.

采用由乙酸乙酯 - 正丁醇 - 水（2:7:9，v/v/v）组成的两相溶剂系统的制备型高速逆流色谱（HSCCC），成功地从传统中药板蓝根中分离出了马蓝苷B和靛玉红苷A。从250 mg粗提物（其中马蓝苷B含量为24.8%，靛玉红苷A含量为28.4%）中一步洗脱，通过高效液相色谱（HPLC）测定，共获得了59.2 mg纯度为94.6%的马蓝苷B和66.1 mg纯度为99.0%的靛玉红苷A，马蓝苷B和靛玉红苷A的回收率分别为90.3%和92.2%。通过红外光谱（IR）、质谱（MS）、氢核磁共振（1H NMR）和碳核磁共振（13C NMR）对其化学结构进行了鉴定。