College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
Molecules. 2012 Jul 27;17(8):9002-9. doi: 10.3390/molecules17089002.
A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3β-hydroxy-8β-[4'-hydroxytigloyloxy]-costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and ¹H-NMR.
建立了一种高速逆流色谱（HSCCC）方法，用于从菊苣（Eupatorium lindleyanum DC.）中制备分离三种倍半萜内酯。选择的两相溶剂系统由正己烷-乙酸乙酯-甲醇-水（1:4:2:3，v/v/v/v）组成。从菊苣的正丁醇部分 540mg 中，通过一步 HSCCC 分离，获得了 10.8mg 的 3β-羟基-8β-[4'-羟基-4'-甲酰基]-齐墩果烷内酯、17.9mg 的 eupalinolide A 和 19.3mg 的 eupalinolide B，纯度分别为 91.8%、97.9%和 97.1%，通过 HPLC 确定。通过 ESI-MS 和 ¹H-NMR 进一步鉴定了它们的结构。
