Shang H Y, Wei Y M, Long H, Yan Z H, Zheng Y L
Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan 611830, China.
Genetika. 2005 Dec;41(12):1656-64.
Three low-molecular-weight (LMW) glutenin-like genes (designated as Ssy1, Ssy2 and Ssy3) from Secale sylvestre Host were isolated and characterized. The three genes consist of a predicted highly conservative signal peptide with 20 amino acids, a short N-terminal region with 13 amino acids, a highly variable repetitive domain and a less variable C-terminal domain. The deduced amino acid sequences of the three genes were the LMW-m type due to a methionine residue at the N-terminus. The phylogenic analysis indicated that the prolamin genes could be perfectly clustered into five groups, including HMW-GS, LMW-GS, alpha/beta-, gamma- and omega-prolamin. The LMW glutenin-like genes of S. sylvestre were more orthologous with the LMW-GS genes of wheat and B hordein genes of barley, which also had been confirmed by the homology analysis with the LMW-GS of wheat at Glu-A3, Glu-B3 and Glu-D3 loci. These results indicated that a chromosome locus (designated as Glu-R3) might be located on the R genome of S. sylvestre with the functions similar to the Glu-3 locus in wheat and its related species.
从小麦草（Secale sylvestre Host）中分离并鉴定了三个低分子量（LMW）类麦谷蛋白基因（命名为Ssy1、Ssy2和Ssy3）。这三个基因由一个预测的含20个氨基酸的高度保守信号肽、一个含13个氨基酸的短N端区域、一个高度可变的重复结构域和一个可变较小的C端结构域组成。由于N端存在甲硫氨酸残基，这三个基因推导的氨基酸序列属于LMW-m型。系统发育分析表明，醇溶蛋白基因可完美地聚类为五组，包括高分子量谷蛋白亚基（HMW-GS）、低分子量谷蛋白亚基（LMW-GS）、α/β-醇溶蛋白、γ-醇溶蛋白和ω-醇溶蛋白。小麦草的LMW类麦谷蛋白基因与小麦的LMW-GS基因和大麦的B醇溶蛋白基因的直系同源性更高，这也通过与小麦Glu-A3、Glu-B3和Glu-D3位点的LMW-GS进行同源性分析得到了证实。这些结果表明，在小麦草的R基因组上可能存在一个染色体位点（命名为Glu-R3），其功能与小麦及其近缘物种中的Glu-3位点相似。
