Riabchenko L E, Novikov A D, Golyshin P N, Ianenko A S
Genetika. 2005 Dec;41(12):1725-7.
Nucleotide sequence of cryptic plasmid pN30 from a Rhodococcus erythropolis 30 soil isolate was determined. Plasmid DNA consists of 5403 nucleotide pairs and contains about 62% GC pairs, which is typical of Rhodococcus DNA. No significant homology was determined between the pN30 DNA sequence and those of known plasmids. Computer-aided analysis of pN30 sequence revealed open reading frames that encode proteins strongly homologous to replicative proteins encoded by small cryptic plasmids of different actinomycetes.
测定了从红平红球菌30土壤分离株中获得的隐蔽质粒pN30的核苷酸序列。质粒DNA由5403个核苷酸对组成,含有约62%的GC对,这是红球菌属DNA的典型特征。未确定pN30 DNA序列与已知质粒序列之间有显著同源性。对pN30序列的计算机辅助分析揭示了一些开放阅读框,这些开放阅读框编码的蛋白质与不同放线菌的小型隐蔽质粒编码的复制蛋白高度同源。